| WANG Guanghua,DONG Junde,LI Xiang,SUN Huimin. 2010. The bacterial diversity in surface sediment from the South China Sea. Acta Oceanologica Sinica, (4):98－105 |
| The bacterial diversity in surface sediment from the South China Sea |
| The bacterial diversity in surface sediment from the South China Sea |
| Received:April 27, 2009 Revised:December 04, 2009 |
| DOI：10.1007/s13131-010-0054-7 |
| Key words:bacterial diversity DGGE marine sediment South China Sea |
| 中文关键词: bacterial diversity DGGE marine sediment South China Sea |
| 基金项目:The State Principal and Basic Research and Development Program of the Ministry of Sciences and Technology of China under contract No.2010CB833801; the National Supportive Project under contract No. 2006BAB19B02; Hundred Talents Program of Chinese Academy of Science. |
| Author Name | Affiliation | E-mail | | WANG Guanghua | Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou 510301, China
Graduate University of Chinese Academy of Science, Beijing 100049, China | | | DONG Junde | Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou 510301, China | | | LI Xiang | Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou 510301, China | Lixiang@scsio.ac.cn | | SUN Huimin | Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou 510301, China
Graduate University of Chinese Academy of Science, Beijing 100049, China | |
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| Abstract: |
| 16S rDNA sequencing results from this study and literatures demonstrate that sediment bacteria in the South China Sea (SCS) were very diverse, which contained 22 of the 24 phyla of bacteria investigated from marine sediment, however, it was very imbalance among stations. So bacterial diversity from 15 samples which covered a wide range of sediment types from 20 to 3 888 m in depth was studied in DGGE (denature gradient gel electrophoresis) in this paper. The DGGE results indicate that both sediment bacterial diversity and diversity difference among stations were significant. Thirty representative and differential fingerprints among samples were recovered and sequenced, phylogenetic analysis indicates that they may belong to Proteobacteria (α-, β-, γ-, δ-, ε-), Planctomycetes, Firmicutes, Chloroflexi, Acidobacteria, Actinobacteria, Nitrospirae, Gemmatimonadetes, candidate division WS3 and so on, of which, Gemmatimonadetes and candidate division WS3 bacteria were first detected in SCS sediment. This study also shows that bacterial diversity analysis based on DGGE was more potential than traditional 16S rDNA clone library in multiple sample analysis. |
| 中文摘要: |
| 16S rDNA sequencing results from this study and literatures demonstrate that sediment bacteria in the South China Sea (SCS) were very diverse, which contained 22 of the 24 phyla of bacteria investigated from marine sediment, however, it was very imbalance among stations. So bacterial diversity from 15 samples which covered a wide range of sediment types from 20 to 3 888 m in depth was studied in DGGE (denature gradient gel electrophoresis) in this paper. The DGGE results indicate that both sediment bacterial diversity and diversity difference among stations were significant. Thirty representative and differential fingerprints among samples were recovered and sequenced, phylogenetic analysis indicates that they may belong to Proteobacteria (α-, β-, γ-, δ-, ε-), Planctomycetes, Firmicutes, Chloroflexi, Acidobacteria, Actinobacteria, Nitrospirae, Gemmatimonadetes, candidate division WS3 and so on, of which, Gemmatimonadetes and candidate division WS3 bacteria were first detected in SCS sediment. This study also shows that bacterial diversity analysis based on DGGE was more potential than traditional 16S rDNA clone library in multiple sample analysis. |
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