| LIU Bin,WU Suijie,XIE Lianhui. 2010. Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1. Acta Oceanologica Sinica, (3):84－89 |
| Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1 |
| Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1 |
| Received:March 05, 2009 Revised:August 21, 2009 |
| DOI：10.1007/s13131-010-0039-6 |
| Key words:thermophile bacteriophage genome sequence proteomic analysis |
| 中文关键词: thermophile bacteriophage genome sequence proteomic analysis |
| 基金项目:The Key Natural Science Foundation of Fujian under contract No. 2007J0004; the National Natural Science Foundation of China under contract No. 40576076. |
| Author Name | Affiliation | E-mail | | LIU Bin | Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China | liubin618@hotmail.com | | WU Suijie | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | XIE Lianhui | Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China | |
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| Abstract: |
| Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames (ORFs). Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction. |
| 中文摘要: |
| Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames (ORFs). Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction. |
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