| MAO Yong,XU Bing,SU Yongquan,ZHANG Zhiwen,DING Shaoxiong,WANG Ding,WANG Jun. 2010. Cloning and mRNA expression of macrophage migration inhibitory factor (MIF) gene of large yellow croaker (Pseudosciaena crocea). Acta Oceanologica Sinica, (3):63－73 |
| Cloning and mRNA expression of macrophage migration inhibitory factor (MIF) gene of large yellow croaker (Pseudosciaena crocea) |
| Cloning and mRNA expression of macrophage migration inhibitory factor (MIF) gene of large yellow croaker (Pseudosciaena crocea) |
| Received:April 15, 2009 Revised:February 08, 2010 |
| DOI：10.1007/s13131-010-0037-8 |
| Key words:macrophage migration inhibitory factor (MIF) vibriosis large yellow croaker mRNA expression |
| 中文关键词: macrophage migration inhibitory factor (MIF) vibriosis large yellow croaker mRNA expression |
| 基金项目:The National Natural Science Foundation of China under contract No. 40976096; the National Department Public Benefit Research Foundation of China under contract No. 200903029; the National High Technology Research and Development Program of China (863 Program) under contract No. 2006AA10A405. |
| Author Name | Affiliation | E-mail | | MAO Yong | Fisheries College of Guangdong Ocean University, Zhanjiang 524025, China
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | XU Bing | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | SU Yongquan | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | ZHANG Zhiwen | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | DING Shaoxiong | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | WANG Ding | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | WANG Jun | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | junw@xmu.edu.cn |
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| Abstract: |
| Mammalian macrophage migration inhibitory factor (MIF) plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea (LycMIF) using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection. |
| 中文摘要: |
| Mammalian macrophage migration inhibitory factor (MIF) plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea (LycMIF) using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection. |
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