| LI Yongjun,ZHENG Zejun,ZHAO Yulong,WEI Xiaona,ZHU Lin,HUANG Xitai. 2010. A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene. Acta Oceanologica Sinica, (2):93－97 |
| A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene |
| A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene |
| Received:January 03, 2009 Revised:August 20, 2009 |
| DOI：10.1007/s13131-010-0027-x |
| Key words:Vibrio vulnificus LAMP virulence-correlated gene seawater culture-free VBNC |
| 中文关键词: Vibrio vulnificus LAMP virulence-correlated gene seawater culture-free VBNC |
| 基金项目:The National High-Tech Research and Development Program of China ("863") under contract No.2006AA09Z170. |
| Author Name | Affiliation | E-mail | | LI Yongjun | The College of Life Sciences, Nankai University, Tianjin 300071, China | | | ZHENG Zejun | The College of Life Sciences, Nankai University, Tianjin 300071, China | | | ZHAO Yulong | The College of Life Sciences, Nankai University, Tianjin 300071, China | | | WEI Xiaona | The College of Life Sciences, Nankai University, Tianjin 300071, China | | | ZHU Lin | The College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China | | | HUANG Xitai | The College of Life Sciences, Nankai University, Tianjin 300071, China | huangxt@nankai.edu.cn |
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| Abstract: |
| Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters. In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons, based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg). The new assay method allows differentiation between the virulent and non-virulent strain of V. vulnificus accurately. This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method. The results show that the method is sensitive, accurate and convenient. |
| 中文摘要: |
| Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters. In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons, based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg). The new assay method allows differentiation between the virulent and non-virulent strain of V. vulnificus accurately. This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method. The results show that the method is sensitive, accurate and convenient. |
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