| ZHENG Runling,LI Jiale,NIU Donghong. 2010. The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae). Acta Oceanologica Sinica, (2):88－92 |
| The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae) |
| The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae) |
| Received:October 07, 2008 Revised:September 03, 2009 |
| DOI：10.1007/s13131-010-0026-y |
| Key words:Sinonovacula constricta Mitochondrial DNA |
| 中文关键词: Sinonovacula constricta Mitochondrial DNA |
| 基金项目:"863" Hi-tech Research and Development Program of China under contract No. 2006 AA10 A410; Shanghai Leading Academic Discipline Project under contract No. Y1101; Dr. Start-up Fund under contract No. A-2400-09-0144. |
| Author Name | Affiliation | E-mail | | ZHENG Runling | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China | | | LI Jiale | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
Aquaculture Division, E-Institute of Shanghai Universities, Shanghai 201306, China | jlli@shou.edu.cn | | NIU Donghong | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China | |
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| Abstract: |
| The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricta (GenBank accession number EU880278) has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs (bp) are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA (mtDNA) did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A+T (A 25.9%, C 10.5%, G 22.5%, and T 41.1%). This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia genomes. |
| 中文摘要: |
| The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricta (GenBank accession number EU880278) has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs (bp) are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA (mtDNA) did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A+T (A 25.9%, C 10.5%, G 22.5%, and T 41.1%). This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia genomes. |
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