| SHA Zhenxia,REN Guocheng,WANG Xianli,WANG Na,CHEN Songlin. 2010. Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Acta Oceanologica Sinica, (2):81－87 |
| Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis) |
| Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis) |
| Received:December 31, 2008 Revised:September 15, 2009 |
| DOI：10.1007/s13131-010-0025-z |
| Key words:embryonic cell line half smooth tongue sole karyotype GFP LCDV CSEC |
| 中文关键词: embryonic cell line half smooth tongue sole karyotype GFP LCDV CSEC |
| 基金项目:The State "863" High-Technology Research and Development Project of China under contract Nos 2006AA09Z406 and 2006AA10A401; the National Natural Science Foundation of China under contract No. 40376047; the Taishan Scholar Project of Shandong Province and MOA for returned scientists from abroad. |
| Author Name | Affiliation | E-mail | | SHA Zhenxia | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | | | REN Guocheng | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China
College of Biology, Shandong Normal University, Jinan 250014, China | | | WANG Xianli | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China
College of Marine Life, Ocean University of China, Qingdao 266003, China | | | WANG Na | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | | | CHEN Songlin | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | chensl@ysfri.ac.cn |
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| Abstract: |
| A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), 2.5 ng/cm3 bFGF (basic fibroblast growth factor), 1 ng/cm3 LIF (leukemia inhibitory factor) and 50 mmol/dm3 2-ME (2-mecaptoethanol). The cells grew well in the temperature range of 24-30℃ and the optimal growth temperature was 24℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV) in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus. |
| 中文摘要: |
| A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), 2.5 ng/cm3 bFGF (basic fibroblast growth factor), 1 ng/cm3 LIF (leukemia inhibitory factor) and 50 mmol/dm3 2-ME (2-mecaptoethanol). The cells grew well in the temperature range of 24-30℃ and the optimal growth temperature was 24℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV) in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus. |
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