| DU Zongjun,ZHANG Wanyi,XIA Hongjie,LÜ Guoqiang,CHEN Guanjun. 2010. Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones. Acta Oceanologica Sinica, (2):62－69 |
| Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones |
| Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones |
| Received:November 21, 2008 Revised:April 15, 2009 |
| DOI：10.1007/s13131-010-0023-1 |
| Key words:Anthopleura midori Bacterial diversity 16S rRNA gene Phylogeny Identification |
| 中文关键词: Anthopleura midori Bacterial diversity 16S rRNA gene Phylogeny Identification |
| 基金项目:The State Key Laboratory of Microbial Technology under contract No. M2010-04; Independent Innovation Foundation of Shandong University (IIFSDU); the National Natural Science Foundation of China under contract No. 40730847; Special Non-profit Research Project from Ministry of Agriculture of the Peoples Republic of China under contract No. nyhyzx 07-046. |
| Author Name | Affiliation | E-mail | | DU Zongjun | State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
College of Marine Science, Shandong University at Weihai, Weihai 264209, China | | | ZHANG Wanyi | College of Marine Science, Shandong University at Weihai, Weihai 264209, China | | | XIA Hongjie | College of Marine Science, Shandong University at Weihai, Weihai 264209, China | | | LÜ Guoqiang | College of Marine Science, Shandong University at Weihai, Weihai 264209, China | | | CHEN Guanjun | State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
College of Marine Science, Shandong University at Weihai, Weihai 264209, China | guanjun@sdu.edu.cn |
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| Abstract: |
| A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micrococcus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials. |
| 中文摘要: |
| A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micrococcus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials. |
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