| CHAI Yingmei,HUANG Xiaohang,CONG Bailin,LIU Chenlin,LIU Shenghao. 2010. Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus). Acta Oceanologica Sinica, (1):116－119 |
| Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus) |
| Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus) |
| Received:December 11, 2008 Revised:July 13, 2009 |
| DOI：10.1007/s13131-010-0014-2 |
| Key words:patch-clamp technique potassium channel turbot peripheral blood lymphocyte |
| 中文关键词: patch-clamp technique potassium channel turbot peripheral blood lymphocyte |
| 基金项目:The National Natural Science Foundation of China under contract Nos 40476060 and 40876089; the State Key Basic Research Program under contract No. 2006CB101805. |
| Author Name | Affiliation | E-mail | | CHAI Yingmei | College of Marine Life Sciences, Shandong University at Weihai, Weihai 264209, China
Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | HUANG Xiaohang | Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | xiaohanghuang@fio.org.cn | | CONG Bailin | Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | LIU Chenlin | Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | LIU Shenghao | Marine Bioactive Substances Laboratory, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | |
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| Abstract: |
| In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot (Scophthalmus maximus). Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dm3 TEA or 5 mmol/dm3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot. |
| 中文摘要: |
| In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot (Scophthalmus maximus). Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dm3 TEA or 5 mmol/dm3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot. |
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