| MA Yuexin,LIU Pengliang,ZHANG Yongsheng,CAO Shanmao,LI Dantong,CHEN Wei. 2010. Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4. Acta Oceanologica Sinica, (1):69－78 |
| Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4 |
| Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4 |
| Received:February 16, 2009 Revised:August 19, 2009 |
| DOI：10.1007/s13131-010-0009-z |
| Key words:Pseudoalteromonas antifouling bacterium Ulva pertusa biofouling anti-algal activity |
| 中文关键词: Pseudoalteromonas antifouling bacterium Ulva pertusa biofouling anti-algal activity |
| 基金项目:The Natural Science Foundation of Liaoning Province under contract No. 20062129. |
| Author Name | Affiliation | E-mail | | MA Yuexin | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China | mayuexin@dlfu.edu.cn | | LIU Pengliang | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China | | | ZHANG Yongsheng | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China | | | CAO Shanmao | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China | | | LI Dantong | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China | | | CHEN Wei | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China | |
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| Abstract: |
| The effect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of the antifouling substance in the supernatant of CI4, the culture supernatants were tested for activity after heat treatment, enzymatic treatments, size fractionation, and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with specific activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive effect on spore germination. Pronase and carboxypeptidase y did not significantly affect the activity of inhibitory component, suggesting that the component was not a protein or a peptide. The bacterium CI4 was identified as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analysis. |
| 中文摘要: |
| The effect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of the antifouling substance in the supernatant of CI4, the culture supernatants were tested for activity after heat treatment, enzymatic treatments, size fractionation, and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with specific activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive effect on spore germination. Pronase and carboxypeptidase y did not significantly affect the activity of inhibitory component, suggesting that the component was not a protein or a peptide. The bacterium CI4 was identified as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analysis. |
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