| MA Ying,WANG Zhiyong,CHEN Zhengqiang,LU Yu,WANG Lin. 2009. Phylogenetic diversity of bacterial biofilms covering the settlement substrates of nona-porous abalones(Haliotis diversicolor supertexta). Acta Oceanologica Sinica, (5):94－102 |
| Phylogenetic diversity of bacterial biofilms covering the settlement substrates of nona-porous abalones(Haliotis diversicolor supertexta) |
| Phylogenetic diversity of bacterial biofilms covering the settlement substrates of nona-porous abalones(Haliotis diversicolor supertexta) |
| Received:August 26, 2008 Revised:March 18, 2009 |
| DOI： |
| Key words:nona-porous abalone(Haliotis diversicolor supertexta) settlement substrate bacterial biofilm 16S rRNA |
| 中文关键词: nona-porous abalone(Haliotis diversicolor supertexta) settlement substrate bacterial biofilm 16S rRNA |
| 基金项目:The Natural Science Foundation of Fujian Province of China under contract Nos 2005k055 and B0740012;the Innovative Research Team under contract No 2006A001;Science Foundation of Jimei University, China |
| Author Name | Affiliation | E-mail | | MA Ying | The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China | WANG Zhiyong,E-mail:zywang@jmu.edu.cn | | WANG Zhiyong | The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China | | | CHEN Zhengqiang | The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China | | | LU Yu | The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China | | | WANG Lin | The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China | |
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| Abstract: |
| The settlement substrates of nona-porous abalones (Haliotis diversicolor supertexta) are covered with biofilms in which several types of microorganisms coexist and interact.These microorganisms are usually important causes of juvenile abalone disease as well as organisms useful in promoting abalones' adhesion.The bacterial community structure of the biofilms remains unclear.The aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria.Total DNA of bacteria in biofilms was extracted,and 16S rRNA gene clone library was constructed using the primers specific for the domain bacteria.Subsequently,30 randomly selected positive clones were screened by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis,and resulted in 15 different RFLP patterns.Sequences analysis of representatives from each unique RFLP type revealed high genetic diversity in the bacterial populations.These sequences fell into nine major lineages of the bacterial domains:α-,β-,γ- and δ-subdivisions of the Proteobacteria; Planctomycete,Actinobacteria,Firmicutes,Verrucomicrobium spp.,and CytophagaFlexibacter-Bacteroides spp.Phylogenetic analysis indicated that the dominant phylotypes were most closely related to environmental and clinical Burkholderia cepacia of the β-Proteobacteria,and Roseobacteria of the α-Proteobacteria. |
| 中文摘要: |
| The settlement substrates of nona-porous abalones (Haliotis diversicolor supertexta) are covered with biofilms in which several types of microorganisms coexist and interact.These microorganisms are usually important causes of juvenile abalone disease as well as organisms useful in promoting abalones' adhesion.The bacterial community structure of the biofilms remains unclear.The aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria.Total DNA of bacteria in biofilms was extracted,and 16S rRNA gene clone library was constructed using the primers specific for the domain bacteria.Subsequently,30 randomly selected positive clones were screened by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis,and resulted in 15 different RFLP patterns.Sequences analysis of representatives from each unique RFLP type revealed high genetic diversity in the bacterial populations.These sequences fell into nine major lineages of the bacterial domains:α-,β-,γ- and δ-subdivisions of the Proteobacteria; Planctomycete,Actinobacteria,Firmicutes,Verrucomicrobium spp.,and CytophagaFlexibacter-Bacteroides spp.Phylogenetic analysis indicated that the dominant phylotypes were most closely related to environmental and clinical Burkholderia cepacia of the β-Proteobacteria,and Roseobacteria of the α-Proteobacteria. |
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