| YANG Bo,OUYANG Jianping,AO Jingqun,CHEN Xinhua. 2009. A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China. Acta Oceanologica Sinica, (1):62－71 |
| A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China |
| A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China |
| Received:September 10, 2007 Revised:August 30, 2008 |
| DOI： |
| Key words:Xiamen sea hot springs thermophilic bacteria moderately thermophilic acidophiles cultiva-ble microbial community |
| 中文关键词: Xiamen sea hot springs thermophilic bacteria moderately thermophilic acidophiles cultiva-ble microbial community |
| 基金项目:The Scientific Research Project of Marine Public Welfare Industry of China under contract No. 200805032; China Ocean Mineral Re-sources R & D Association under contract No. DYXM-115-02-2-07; the National Infrastructure of Natural Resources for Science and Technology Program of China under contract No. 2005DKA21209. |
| Author Name | Affiliation | E-mail | | YANG Bo | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administra-tion, Xiamen 361005, China | | | OUYANG Jianping | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administra-tion, Xiamen 361005, China | | | AO Jingqun | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administra-tion, Xiamen 361005, China | | | CHEN Xinhua | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administra-tion, Xiamen 361005, China | Chenxinh@tom.com |
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| Abstract: |
| Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culture-dependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55-90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8-3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sul-f obacillus acidophilus (Strain 9), and Sulf obacillus thermosulf idooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene se-quence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community. |
| 中文摘要: |
| Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culture-dependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55-90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8-3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sul-f obacillus acidophilus (Strain 9), and Sulf obacillus thermosulf idooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene se-quence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community. |
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