| GAO Shan,HU Xiaozhong,CHEN Zigui,XU Henglong,YI Zhenzhen,LIN Xiaofeng,LI Jiqiu. 2009. Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA (RAPD). Acta Oceanologica Sinica, (1):55－61 |
| Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA (RAPD) |
| Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA (RAPD) |
| Received:October 24, 2007 Revised:May 22, 2008 |
| DOI： |
| Key words:marine plankton RAPD community diversity phylogeny |
| 中文关键词: marine plankton RAPD community diversity phylogeny |
| 基金项目:The National Natural Science Foundation of China under contract Nos 30570236, U0633006, 40506033 and 40676076; the Program for New Century Excellent Talents in Universities of China under contract No. NCET 05-0595. |
| Author Name | Affiliation | E-mail | | GAO Shan | Laboratory of Protozoology, Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China | | | HU Xiaozhong | Laboratory of Protozoology, Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China | xiaozhonghu@ouc.edu.cn | | CHEN Zigui | Laboratory of Protozoology, Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China | | | XU Henglong | Laboratory of Protozoology, Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China | | | YI Zhenzhen | Laboratory of Protozoology, Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China | | | LIN Xiaofeng | Laboratory of Protozoology, College of Life Science, South China Normal University, Guangzhou 510631, China | | | LI Jiqiu | Laboratory of Protozoology, College of Life Science, South China Normal University, Guangzhou 510631, China | |
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| Abstract: |
| Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that:(1) the band sharing index values were in the range of 0.504 2-0.763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well. |
| 中文摘要: |
| Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that:(1) the band sharing index values were in the range of 0.504 2-0.763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well. |
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