| WANG Li,SHI Dawei,WU Xinzhong. 2008. Characterization and expression analysis of an allograft inflammatory factor-1 homologue in yellow grouper (Epinephelus awoara). Acta Oceanologica Sinica, (6):141－148 |
| Characterization and expression analysis of an allograft inflammatory factor-1 homologue in yellow grouper (Epinephelus awoara) |
| Characterization and expression analysis of an allograft inflammatory factor-1 homologue in yellow grouper (Epinephelus awoara) |
| Received:August 14, 2007 Revised:April 15, 2008 |
| DOI： |
| Key words:allograft inflammatory factor-1 epinephelus awoara expression |
| 中文关键词: allograft inflammatory factor-1 epinephelus awoara expression |
| 基金项目:The National Natural Science Foundation of China under contract No. 30671619. |
| Author Name | Affiliation | E-mail | | WANG Li | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China | | | SHI Dawei | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | | | WU Xinzhong | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | wuxz@zju.edu.cn |
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| Abstract: |
| Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic calcium-binding protein involved in inflammatory response-related diseases in mammals. Previously an identified AIF-1 gene was simply reported in yellow grouper. The characterization of AIF-1 gene and its expression at the gene and protein level are further described. Yellow grouper AIF-1 is composed of 147 amino acids, and 64%~84% identical to other homologues. Basal level AIF-1 mRNA expression was noted in spleen, anterior kidney and kidney, using reverse-transcription polymerase chain reaction (RT-PCR). After stimulation of LPS, the AIF-1 mRNA expression was up-regulated in tissues examined:spleen, anterior kidney, kidney, heart and liver, but not in muscle. The recombinant AIF-1 protein was expressed in Escherichia coli, and then purified for the development of antiserum. Western blotting analysis revealed a band with a molecular mass of about 17 ku. |
| 中文摘要: |
| Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic calcium-binding protein involved in inflammatory response-related diseases in mammals. Previously an identified AIF-1 gene was simply reported in yellow grouper. The characterization of AIF-1 gene and its expression at the gene and protein level are further described. Yellow grouper AIF-1 is composed of 147 amino acids, and 64%~84% identical to other homologues. Basal level AIF-1 mRNA expression was noted in spleen, anterior kidney and kidney, using reverse-transcription polymerase chain reaction (RT-PCR). After stimulation of LPS, the AIF-1 mRNA expression was up-regulated in tissues examined:spleen, anterior kidney, kidney, heart and liver, but not in muscle. The recombinant AIF-1 protein was expressed in Escherichia coli, and then purified for the development of antiserum. Western blotting analysis revealed a band with a molecular mass of about 17 ku. |
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