| CHU Wuying,YU Lian,QIAN Ronghua,MENG Tao,ZHOU Ruixue,FU Guihong,CHEN Jia,ZHANG Jianshe. 2008. Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea. Acta Oceanologica Sinica, (5):126－133 |
| Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea |
| Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea |
| Received:January 23, 2007 Revised:November 12, 2007 |
| DOI： |
| Key words:globin intergenic intragenic intron large yellow croaker promoter regulation |
| 中文关键词: globin intergenic intragenic intron large yellow croaker promoter regulation |
| 基金项目:The National Natural Science Foundation of China under contract Nos 30640015 and 30771644, and the Major Program of Zhejiang Province of China under contract No. 2005C23085. |
| Author Name | Affiliation | E-mail | | CHU Wuying | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China | | | YU Lian | Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China | | | QIAN Ronghua | Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China | | | MENG Tao | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | ZHOU Ruixue | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | FU Guihong | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | CHEN Jia | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | ZHANG Jianshe | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | jzhang@ccsu.cn |
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| Abstract: |
| One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the αand β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex, the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were transiently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements (PRE) located in the β-globin gene intron 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked αand β-globin genes. |
| 中文摘要: |
| One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the αand β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex, the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were transiently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements (PRE) located in the β-globin gene intron 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked αand β-globin genes. |
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