| CHANG Yumei,DING Lei,LI Mingyun,XUE Liangyi,LIANG Liqun,HE Jianguo,LEI Qingquan. 2008. Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers. Acta Oceanologica Sinica, (4):156－164 |
| Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers |
| Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers |
| Received:October 12, 2007 Revised:April 09, 2008 |
| DOI： |
| Key words:Pseudosciaena crocea microsatellite marker bred stock germplasm admix |
| 中文关键词: Pseudosciaena crocea microsatellite marker bred stock germplasm admix |
| 基金项目:The National High Technology Research and Development Program of China under contract No. 2006AA10A405. |
| Author Name | Affiliation | E-mail | | CHANG Yumei | Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
College of Life Sciences, Zhongshan University, Guangzhou 510275, China | | | DING Lei | Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
Life Science of Technology Institute, Dalian Fisheries University, Dalian 116023, China | | | LI Mingyun | Life Science and Biotechnology Faculty, Ningbo University, Ningbo 315211, China | | | XUE Liangyi | Life Science and Biotechnology Faculty, Ningbo University, Ningbo 315211, China | | | LIANG Liqun | Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin 150080, China | llq-1019@163.com | | HE Jianguo | College of Life Sciences, Zhongshan University, Guangzhou 510275, China | | | LEI Qingquan | College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin 150080, China | |
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| Abstract: |
| The germplasm of breeding large yellow croaker (Pseudosciaena crocea Richardson) was revealed using 12 microsatellite markers. The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min-Yue stocks and two hybrid groups, as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity. The value of pairwise differentiation coefficient(FST) was only 13.1% between Daiqu and Min-Yue stocks, demonstrating the low level of differentiation and a close relationship. However, STRUCTURE simulations and phylogenetic tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures. Examinations of individual admixture showed that Min-Yue stock had been contaminated by alien individuals. Moreover, the genetic structures of the two hybrid groups resembled those of their parents, especially affected more by their female parents. Finally, the values of average observed heterozygosity between parents and their ascendants were compared and tested, as a result of no detectable differences (P > 0.05). |
| 中文摘要: |
| The germplasm of breeding large yellow croaker (Pseudosciaena crocea Richardson) was revealed using 12 microsatellite markers. The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min-Yue stocks and two hybrid groups, as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity. The value of pairwise differentiation coefficient(FST) was only 13.1% between Daiqu and Min-Yue stocks, demonstrating the low level of differentiation and a close relationship. However, STRUCTURE simulations and phylogenetic tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures. Examinations of individual admixture showed that Min-Yue stock had been contaminated by alien individuals. Moreover, the genetic structures of the two hybrid groups resembled those of their parents, especially affected more by their female parents. Finally, the values of average observed heterozygosity between parents and their ascendants were compared and tested, as a result of no detectable differences (P > 0.05). |
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