| ZHU Yanbing,WU Chenglin,YANG Feng. 2008. White spot syndrome virus envelope protein VP124 involved in the virus infection. Acta Oceanologica Sinica, (4):130－136 |
| White spot syndrome virus envelope protein VP124 involved in the virus infection |
| White spot syndrome virus envelope protein VP124 involved in the virus infection |
| Received:August 20, 2007 Revised:October 08, 2007 |
| DOI： |
| Key words:white spot syndrome virus envelope protein VP124 antibody infection neutralization assay |
| 中文关键词: white spot syndrome virus envelope protein VP124 antibody infection neutralization assay |
| 基金项目:The "863" Program of China under contract No. 2003AA626020 and the Fujian Science Fund of Chima under contract No. 2003F001. |
| Author Name | Affiliation | E-mail | | ZHU Yanbing | School of Biotechnology Engineering, Jimei University, Xiamen 361021, China | yanbingzhu@163.com | | WU Chenglin | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | YANG Feng | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | |
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| Abstract: |
| White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually. And then they were injected intramuscularly into crayfish (Procambarus clarkii) to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection. |
| 中文摘要: |
| White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually. And then they were injected intramuscularly into crayfish (Procambarus clarkii) to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection. |
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