| YI Jiabo,CHEN Qiang,ZOU Wenzheng,YAN Qingpi,ZHUANG Zhixia,WANG Xiaoru. 2008. Starvation effects on pathogenic Vibrio alginolyticus in natural seawater. Acta Oceanologica Sinica, (4):120－129 |
| Starvation effects on pathogenic Vibrio alginolyticus in natural seawater |
| Starvation effects on pathogenic Vibrio alginolyticus in natural seawater |
| Received:April 20, 2007 Revised:November 06, 2007 |
| DOI： |
| Key words:Vibrio alginolyticus starvation survival adhesion ELISA resistance |
| 中文关键词: Vibrio alginolyticus starvation survival adhesion ELISA resistance |
| 基金项目:The Hi-Tech Research and Development Program ("863" Program)of China under contract Nos 2001AA635070 and 2002AA639600 and the Natural Science Foundation of Fujian Province of China under contract No. B0410022. |
| Author Name | Affiliation | E-mail | | YI Jiabo | Fisheries College, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen 361021, China | | | CHEN Qiang | Fisheries College, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen 361021, China | | | ZOU Wenzheng | Fisheries College, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen 361021, China | | | YAN Qingpi | Fisheries College, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen 361021, China | yanqp@jmu.edu.cn | | ZHUANG Zhixia | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | WANG Xiaoru | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | |
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| Abstract: |
| To get a better understanding of the starvation survival strategy of pathogenic Vibrio alginolyticus, log-phase cells were inoculated into sterile natural seawater for starvation studies. The results showed that all of total bacteria number, viable bacteria number and CFU number of V. alginolyticus increased remarkably at the initial starvation stage; after reaching their peaks at 5 d, both total bacteria number and viable bacteria number of V. alginolyticus fell slowly, while the CFU number fell more quickly after reaching its peak at 10 d; V. alginolyticus elongated their cells at the prophase of starvation, and then shrunk their volume and turned their shapes into ovals from rods at the anaphase of starvation; starved cells showed more sensitivity to heating and UV; starved cells showed no significant difference from unstarved ones at the lowest detection limit determined by indirect enzyme-linked immunosorbent assay (ELISA); starved cells' ability to adhere to the skin mucus of large yellow croaker (Pseudosciaena crocea) showed a sharp decline as the starvation time increases; the cellular protein of V. alginolyticus increased remarkably at the anaphase of starvation. The results indicated that pathogenic V. alginolyticus could survive in starvation for relatively long periods of time (≥ 2 months) in 28℃ natural seawater due to the morphological and physiological changes; however, starved V. alginolyticus cells showed less virulence and higher sensitivity under environmental stresses. |
| 中文摘要: |
| To get a better understanding of the starvation survival strategy of pathogenic Vibrio alginolyticus, log-phase cells were inoculated into sterile natural seawater for starvation studies. The results showed that all of total bacteria number, viable bacteria number and CFU number of V. alginolyticus increased remarkably at the initial starvation stage; after reaching their peaks at 5 d, both total bacteria number and viable bacteria number of V. alginolyticus fell slowly, while the CFU number fell more quickly after reaching its peak at 10 d; V. alginolyticus elongated their cells at the prophase of starvation, and then shrunk their volume and turned their shapes into ovals from rods at the anaphase of starvation; starved cells showed more sensitivity to heating and UV; starved cells showed no significant difference from unstarved ones at the lowest detection limit determined by indirect enzyme-linked immunosorbent assay (ELISA); starved cells' ability to adhere to the skin mucus of large yellow croaker (Pseudosciaena crocea) showed a sharp decline as the starvation time increases; the cellular protein of V. alginolyticus increased remarkably at the anaphase of starvation. The results indicated that pathogenic V. alginolyticus could survive in starvation for relatively long periods of time (≥ 2 months) in 28℃ natural seawater due to the morphological and physiological changes; however, starved V. alginolyticus cells showed less virulence and higher sensitivity under environmental stresses. |
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