| WANG Wei,YOU Feng,XU Jianhe,SUN Wei,ZHU Xiangping,GAO Tianxiang,ZHANG Peijun. 2008. Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers. Acta Oceanologica Sinica, (2):149－156 |
| Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers |
| Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers |
| Received:April 02, 2007 Revised:August 10, 2007 |
| DOI： |
| Key words:Paralichthys olivaceus meiogynogenesis mitogynogenesis microsatellite-centromere mapping |
| 中文关键词: Paralichthys olivaceus meiogynogenesis mitogynogenesis microsatellite-centromere mapping |
| 基金项目:The National Natural Science Foundation of China under contract Nos 30271036 and 30571445;the National "863" High Technology Research Foundation of China under contract No.2006AA10A404;the Shandong Province Natural Science Foundation of China. |
| Author Name | Affiliation | E-mail | | WANG Wei | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China | | | YOU Feng | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | youfeng@ms.qdio.ac.cn | | XU Jianhe | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | SUN Wei | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | ZHU Xiangping | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | GAO Tianxiang | College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China | | | ZHANG Peijun | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | |
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| Abstract: |
| Twenty microsatellite markers (Po1, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, kop6, kop7, kop8, kop12, kop15, kop18, kop21, kop22, kop23, kop26, Po-str1) were used to assess the meiogynogenetic and mitogynogenetic stocks of the left-eyed flounder, Paralichthys olivaceus, which were derived from single pair crossing.Twelve of the 20 loci utilized showed heterozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder.Microsatellite-centromere map distance, calculated under the assumption of complete interference, ranged from 15.8 cM for kop22 to 50 cM for Po13, Po56 and Po89.Excluding the kop22, the heterozygosities of the rest of the loci were close to 100%, suggesting the occurrence of near complete interference on the chromosome arms that carried these loci.In the mitogynogenetic diploid flounder, each individual showed exact homozygousity and the segregation profiles did not deviate from the Mendelian 1:1 pattern.The results indicated that there was no lethal gene linked with the loci analyzed.Such high interference accounted for the high recombination rates and large map distances.The Po13 and Po56 loci, Po91 and kop18 loci, kop15 and kop21 loci are tightly linked on the same chromosome arm in pairs. |
| 中文摘要: |
| Twenty microsatellite markers (Po1, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, kop6, kop7, kop8, kop12, kop15, kop18, kop21, kop22, kop23, kop26, Po-str1) were used to assess the meiogynogenetic and mitogynogenetic stocks of the left-eyed flounder, Paralichthys olivaceus, which were derived from single pair crossing.Twelve of the 20 loci utilized showed heterozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder.Microsatellite-centromere map distance, calculated under the assumption of complete interference, ranged from 15.8 cM for kop22 to 50 cM for Po13, Po56 and Po89.Excluding the kop22, the heterozygosities of the rest of the loci were close to 100%, suggesting the occurrence of near complete interference on the chromosome arms that carried these loci.In the mitogynogenetic diploid flounder, each individual showed exact homozygousity and the segregation profiles did not deviate from the Mendelian 1:1 pattern.The results indicated that there was no lethal gene linked with the loci analyzed.Such high interference accounted for the high recombination rates and large map distances.The Po13 and Po56 loci, Po91 and kop18 loci, kop15 and kop21 loci are tightly linked on the same chromosome arm in pairs. |
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