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LU Yongzhong,ZHANG Xuecheng. 2008. Analysis on promoter elements of cpc operon from Arthrospira platensis. Acta Oceanologica Sinica, (1):85－91
Analysis on promoter elements of cpc operon from Arthrospira platensis
Analysis on promoter elements of cpc operon from Arthrospira platensis
Received:December 20, 2006  Revised:October 11, 2007
DOI：
Key words:c-phycocyanin  Smart Race  transcription  promoter  cyanobacterium
中文关键词:  c-phycocyanin  Smart Race  transcription  promoter  cyanobacterium
基金项目:The National Natural Science Foundation of China under contract No.30471317.
Author NameAffiliationE-mail
LU Yongzhong . College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Qingdao University of Science and Technology, Qingdao 266042, China 		 
ZHANG Xuecheng . College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China xczhang@ouc.edu.cn 
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Abstract:
      On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E.coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the -285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ.
中文摘要:
      On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E.coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the -285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ.
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