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ZHANG Lingling,BAO Zhenmin,WANG Jue,WANG Shi,HUANG Xiaoting,HU Xiaoli,HU Jingjie. 2007. Cytogenetic analysis in two scallops (Bivalvia: Pectinidae) by PRINS and PI banding. Acta Oceanologica Sinica, (5):153-158
Cytogenetic analysis in two scallops (Bivalvia: Pectinidae) by PRINS and PI banding
Cytogenetic analysis in two scallops (Bivalvia: Pectinidae) by PRINS and PI banding
Received:September 07, 2006  Revised:March 26, 2007
DOI:
Key words:primed in situ labeling  PI banding  Argopecten irradians  Patinopecten yessoensis
中文关键词:  primed in situ labeling  PI banding  Argopecten irradians  Patinopecten yessoensis
基金项目:
Author NameAffiliationE-mail
ZHANG Lingling Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China  
BAO Zhenmin Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China zmbao@ouc.edu.cn 
WANG Jue Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China  
WANG Shi Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China  
HUANG Xiaoting Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China  
HU Xiaoli Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China  
HU Jingjie Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China  
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Abstract:
      Cytogenetic analysis was performed for the bay scallop (Argopecten irradians Lamarck 1819) and the Japanese scallop (Patinopecten yessoensis Jay 1857) by primed in situ labeling (PRINS) and propidium iodide (PI) banding techniques.The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A.irradians and on two submetacentric pairs in P.yessoensis.The histone H3 gene sites differed in number and location between these two species.The C-band-like patterns revealed by PI staining varied considerably between these two species.A.irradians displayed terminal bands at long arms on all chromosomes, centromeric bands on some pairs and interstitial bands on five pairs.P.yessoensis exhibited only centromeric bands on all chromosomes.These results would contribute to the better understanding of karyotype evolution in A.irradians and P.yessoensis.
中文摘要:
      Cytogenetic analysis was performed for the bay scallop (Argopecten irradians Lamarck 1819) and the Japanese scallop (Patinopecten yessoensis Jay 1857) by primed in situ labeling (PRINS) and propidium iodide (PI) banding techniques.The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A.irradians and on two submetacentric pairs in P.yessoensis.The histone H3 gene sites differed in number and location between these two species.The C-band-like patterns revealed by PI staining varied considerably between these two species.A.irradians displayed terminal bands at long arms on all chromosomes, centromeric bands on some pairs and interstitial bands on five pairs.P.yessoensis exhibited only centromeric bands on all chromosomes.These results would contribute to the better understanding of karyotype evolution in A.irradians and P.yessoensis.
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