| LIU Jingwen,JIAO Nianzhi,CAI Huinong,HONG Huasheng. 2007. Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu. Acta Oceanologica Sinica, (4):123－131 |
| Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu |
| Effects of UVB on the expression of proliferating cell nuclear antigen (PCNA) in dinoflagellate Prorocentrum donghaiense Lu |
| Received:July 24, 2006 Revised:January 11, 2007 |
| DOI： |
| Key words:UVB radiation proliferating cell nuclear antigen (PCNA) dinoflagellate Prorocentrum donghaiense Lu |
| 中文关键词: UVB radiation proliferating cell nuclear antigen (PCNA) dinoflagellate Prorocentrum donghaiense Lu |
| 基金项目: |
| Author Name | Affiliation | E-mail | | LIU Jingwen | Bioengineering School of Jimei University, Xiamen 361021, China
State Key Laboratory of Marine Environmental Sciences of Xiamen University, Xiamen 361005, China | | | JIAO Nianzhi | State Key Laboratory of Marine Environmental Sciences of Xiamen University, Xiamen 361005, China | jiao@xmu.edu.cn | | CAI Huinong | Bioengineering School of Jimei University, Xiamen 361021, China | | | HONG Huasheng | State Key Laboratory of Marine Environmental Sciences of Xiamen University, Xiamen 361005, China | |
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| Abstract: |
| PCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated.Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator.The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA. |
| 中文摘要: |
| PCNA has been considered as a useful marker for the estimation of growth rates of marine phytoplankton at the species level. Since dinoflagellates are noted for having many prokaryotic features in that they are the only eukaryotes to have permanently condensed chromosomes as well as lacking histones and a nucleosome, the sensitivity to UVB radiation and the validity of PCNA as a maker of growth rate in dinoflagellate need to be evaluated.Prorocentrum donghaiense Lu was investigated to valuate the UVB sensitivity in relation to cellular and molecular aspects of PCNA as a growth indicator.The effects of UVB radiation on PCNA were studied using the methods of western blots technology and whole-cell immunoflurescence with one mono-antibody. UVB changed the cell morphology, halted the growth and increased the cell size, even caused cell death to a certain extent after treatment with UVB radiation in P. donghaiense. Compared with the control, treating the algal cultures in exponential phases with UVB radiation for 24 h caused chromatin release and increases in protein levels of PCNA. |
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