| ZENG Yinxin,LI Huirong,YU Yong,CHEN Bo,ZHENG Tianling. 2007. Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans. Acta Oceanologica Sinica, (4):93－103 |
| Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans |
| Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans |
| Received:April 14, 2006 Revised:July 22, 2006 |
| DOI： |
| Key words:bacterioplankton diversity arctic antarctic |
| 中文关键词: bacterioplankton diversity arctic antarctic |
| 基金项目: |
| Author Name | Affiliation | E-mail | | ZENG Yinxin | School of Life Sciences, Xiamen University, Xiamen 361005, China
Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China | yxzeng@sina.com | | LI Huirong | Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China | | | YU Yong | Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China | | | CHEN Bo | Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, State Oceanic Administration, Shanghai 200136, China | | | ZHENG Tianling | School of Life Sciences, Xiamen University, Xiamen 361005, China | |
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| Abstract: |
| A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and β-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSw10175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus Shewanella dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria (>99%), but also between polar marine bacteria and bacteria from other aquatic environments (≥ 98.8%), including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. |
| 中文摘要: |
| A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and β-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSw10175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus Shewanella dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria (>99%), but also between polar marine bacteria and bacteria from other aquatic environments (≥ 98.8%), including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. |
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