| HE Shanying,YU Zhigang,MI Tiezhu. 2007. Development of a real-time PCR method for Thalassiosira rotula rapid detection. Acta Oceanologica Sinica, (2):133－139 |
| Development of a real-time PCR method for Thalassiosira rotula rapid detection |
| Development of a real-time PCR method for Thalassiosira rotula rapid detection |
| Received:March 07, 2006 Revised:November 01, 2006 |
| DOI： |
| Key words:red tide Thalassiosira rotula fluorescent quantitative PCR 18S rDNA |
| 中文关键词: red tide Thalassiosira rotula fluorescent quantitative PCR 18S rDNA |
| 基金项目: |
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| Abstract: |
| Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula.A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula.The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. |
| 中文摘要: |
| Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula.A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula.The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. |
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