| HUANG Xiaohang,LAM Patrick P L,LIN Xuezheng,LIU Chenlin,BIAN Ji,GAISANO Herbert. 2007. Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus. Acta Oceanologica Sinica, (1):159－164 |
| Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus |
| Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus |
| Received:January 05, 2006 Revised:May 08, 2006 |
| DOI： |
| Key words:SNARE proteins exocytosis secretion fish |
| 中文关键词: SNARE proteins exocytosis secretion fish |
| 基金项目: |
| Author Name | Affiliation | E-mail | | HUANG Xiaohang | Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | xiaohanghuang@fio.org.cn | | LAM Patrick P L | Department of Physiology and Medicine, University of Toronto, Toronto, M5S 1A8, Canada | | | LIN Xuezheng | Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | LIU Chenlin | Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | BIAN Ji | Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | GAISANO Herbert | Department of Physiology and Medicine, University of Toronto, Toronto, M5S 1A8, Canada | |
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| Abstract: |
| SNARE proteins are a group of membrane-associated proteins involved in exocytosis, secretion and membrane trafficking events in eukaryotic cells.Research on SNARE protein biology has become a more attractive field in recent years, which is applied to marine biology specifically to the fish Tilapia (Oreochromis niloticus).Plasma membrane fractions of different tissues of Tilapia, including brain, liver-pancreas, intestine, skin and muscle, were extracted, and immuno-decorated with isoform-specific antibodies to the SNARE families and associated proteins.The presence of Syntaxins -1A, 2 and 3, SNAP-23 and SNAP-25, VAMP-2, Munc-18-1 and Munc-13 in the brain was identified, which were differentially distributed in the other organ tissues of the fish Tilapia.The distinct distribution of SNARE and associated proteins will serve as the basis for further investigation into their special secretory function in these tissues of the fish. |
| 中文摘要: |
| SNARE proteins are a group of membrane-associated proteins involved in exocytosis, secretion and membrane trafficking events in eukaryotic cells.Research on SNARE protein biology has become a more attractive field in recent years, which is applied to marine biology specifically to the fish Tilapia (Oreochromis niloticus).Plasma membrane fractions of different tissues of Tilapia, including brain, liver-pancreas, intestine, skin and muscle, were extracted, and immuno-decorated with isoform-specific antibodies to the SNARE families and associated proteins.The presence of Syntaxins -1A, 2 and 3, SNAP-23 and SNAP-25, VAMP-2, Munc-18-1 and Munc-13 in the brain was identified, which were differentially distributed in the other organ tissues of the fish Tilapia.The distinct distribution of SNARE and associated proteins will serve as the basis for further investigation into their special secretory function in these tissues of the fish. |
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