| LIU Xiangquan,BAO Zhenmin,HU Jingjie,WANG Shi,ZHAN Aibin,LIU Hui,FANG Jianguang,WANG Rucai. 2007. AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam(Ruditapes philippinarum) in China. Acta Oceanologica Sinica, (1):150－158 |
| AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam(Ruditapes philippinarum) in China |
| AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam(Ruditapes philippinarum) in China |
| Received:January 30, 2006 Revised:November 23, 2006 |
| DOI： |
| Key words:Ruditapes philippinarum genetic diversity geographical population AFLP |
| 中文关键词: Ruditapes philippinarum genetic diversity geographical population AFLP |
| 基金项目: |
| Author Name | Affiliation | E-mail | | LIU Xiangquan | Laboratory of Marine Genetics and Breeding(MGB), Ocean University of China, Qingdao 266003, China | | | BAO Zhenmin | Laboratory of Marine Genetics and Breeding(MGB), Ocean University of China, Qingdao 266003, China | zmbao@ouc.edu.cn | | HU Jingjie | Laboratory of Marine Genetics and Breeding(MGB), Ocean University of China, Qingdao 266003, China | | | WANG Shi | Laboratory of Marine Genetics and Breeding(MGB), Ocean University of China, Qingdao 266003, China | | | ZHAN Aibin | Laboratory of Marine Genetics and Breeding(MGB), Ocean University of China, Qingdao 266003, China | | | LIU Hui | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China | | | FANG Jianguang | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China | | | WANG Rucai | Laboratory of Marine Genetics and Breeding(MGB), Ocean University of China, Qingdao 266003, China | |
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| Abstract: |
| The amplified fragment length polymorphism (AFLP) technology was used to analyze the genetic diversities in four natural populations of Manila clam (Ruditapes philippinarum), distributed in four sea areas of China, i.e., the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea.Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity.The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon's information index was from 0.256 8 to 0.275 6.By analyzing molecular variance (AMOVA), it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas.Cluster analysis by Nei's pairwise distances grouped specimens by geographical origin, except the population of Qingdao.A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations.The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao. |
| 中文摘要: |
| The amplified fragment length polymorphism (AFLP) technology was used to analyze the genetic diversities in four natural populations of Manila clam (Ruditapes philippinarum), distributed in four sea areas of China, i.e., the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea.Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity.The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon's information index was from 0.256 8 to 0.275 6.By analyzing molecular variance (AMOVA), it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas.Cluster analysis by Nei's pairwise distances grouped specimens by geographical origin, except the population of Qingdao.A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations.The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao. |
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