| WANG Shifeng,DU Jiaying,WANG Jun,DING Shaoxiong. 2007. Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers. Acta Oceanologica Sinica, (1):122－129 |
| Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers |
| Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers |
| Received:June 28, 2006 Revised:November 02, 2006 |
| DOI： |
| Key words:Epinephelus malabaricus Epinephelus coioides hybridize molecular identification |
| 中文关键词: Epinephelus malabaricus Epinephelus coioides hybridize molecular identification |
| 基金项目: |
| Author Name | Affiliation | E-mail | | WANG Shifeng | College of Oceanography and Environment Science, Xiamen University, Xiamen 361005, China | | | DU Jiaying | College of Oceanography and Environment Science, Xiamen University, Xiamen 361005, China | | | WANG Jun | College of Oceanography and Environment Science, Xiamen University, Xiamen 361005, China | | | DING Shaoxiong | College of Oceanography and Environment Science, Xiamen University, Xiamen 361005, China | sxding@xmu.edu.cn |
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| Abstract: |
| Using multi-molecular marker technologies and based on morphological criteria, the genetic relationship between Epinepheelus malabaricus and E.coioides was examined in the hope of resolving the long-standing issue of identifying these two species.Results showed that:(1) E.coioides and E.malabaricus should be identified as two species, the consistency of mitochondrial DNA cytochrome b gene sequence between E.malabaricus and E.coioides is 94.4%, and the genetic similarity by AFLP was 0.753 9; (2) Hybridization exists between E.malabaricus and E.coioides, the specific RAPD and AFLP fragments are found to be useful in the identification of these two species, and the genetic properties (both with exterior and inheritance) of hybrid is significantly biased to the male parents; and (3) AFLP was a potentially powerful tool in constructing the genetic linkage map for these two groupers. |
| 中文摘要: |
| Using multi-molecular marker technologies and based on morphological criteria, the genetic relationship between Epinepheelus malabaricus and E.coioides was examined in the hope of resolving the long-standing issue of identifying these two species.Results showed that:(1) E.coioides and E.malabaricus should be identified as two species, the consistency of mitochondrial DNA cytochrome b gene sequence between E.malabaricus and E.coioides is 94.4%, and the genetic similarity by AFLP was 0.753 9; (2) Hybridization exists between E.malabaricus and E.coioides, the specific RAPD and AFLP fragments are found to be useful in the identification of these two species, and the genetic properties (both with exterior and inheritance) of hybrid is significantly biased to the male parents; and (3) AFLP was a potentially powerful tool in constructing the genetic linkage map for these two groupers. |
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