| SU Feng,ZHANG Shicui,YANG Ming,LI Hongyan. 2006. In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus. Acta Oceanologica Sinica, (5):135－140 |
| In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus |
| In vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cells, the gill cell line of flounder Paralichthy olivaceus |
| Received:February 28, 2006 Revised:June 27, 2006 |
| DOI： |
| Key words:acetamiprid cell line cytotoxicity flounder |
| 中文关键词: acetamiprid cell line cytotoxicity flounder |
| 基金项目: |
| Author Name | Affiliation | E-mail | | SU Feng | Department of Bioengineering & Pharmaceutics, Qingdao University of Science & Technology, Qingdao 266042, China
Department of Marine Biology, Ocean University of China, Qingdao 266003, China | | | ZHANG Shicui | Department of Marine Biology, Ocean University of China, Qingdao 266003, China | sczhang@ouc.edu.cn | | YANG Ming | Department of Marine Biology, Ocean University of China, Qingdao 266003, China | | | LI Hongyan | Department of Marine Biology, Ocean University of China, Qingdao 266003, China | |
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| Abstract: |
| The in vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cell line, derived from the gill of flounder Paralichthys olivaceus, was tested by the three widely used endpoint bioassays-neutral red (NR) assay, tetrazolium (MTT) assay and cell protein assay.It was found that acetamiprid was increasingly toxic to FG cells at concentrations of 1 μg/cm3 or above, and the inhibitory concentration 50% values for NR, MTT, and cell protein assays were 38.38, 36.27 and 32.03 μg/cm3, respectively.This appeared to be the first report on the in vitro cytotoxicity of acetamiprid to non-mammalian vertebrate cells.Ultrastructural examination revealed that for the cells exposed to 60 μg/cm3 acetamiprid for 48 h, their mitochondria were severely damaged with the cristae swelled up or disrupted, while their nuclei and rough endoplasmic reticlum (RER) appeared to be still normal.This suggests that mitochondria are possibly the primary target of acetamiprid. |
| 中文摘要: |
| The in vitro acute cytotoxicity of the neonicotinoid insecticide acetamiprid to FG cell line, derived from the gill of flounder Paralichthys olivaceus, was tested by the three widely used endpoint bioassays-neutral red (NR) assay, tetrazolium (MTT) assay and cell protein assay.It was found that acetamiprid was increasingly toxic to FG cells at concentrations of 1 μg/cm3 or above, and the inhibitory concentration 50% values for NR, MTT, and cell protein assays were 38.38, 36.27 and 32.03 μg/cm3, respectively.This appeared to be the first report on the in vitro cytotoxicity of acetamiprid to non-mammalian vertebrate cells.Ultrastructural examination revealed that for the cells exposed to 60 μg/cm3 acetamiprid for 48 h, their mitochondria were severely damaged with the cristae swelled up or disrupted, while their nuclei and rough endoplasmic reticlum (RER) appeared to be still normal.This suggests that mitochondria are possibly the primary target of acetamiprid. |
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