| LI Laihao,XUE Changhu,XUE Yong,LI Zhaojie,FU Xueyan. 2006. The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein. Acta Oceanologica Sinica, (4):124－130 |
| The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein |
| The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein |
| Received:February 12, 2006 Revised:May 30, 2006 |
| DOI： |
| Key words:Laminaria japonica fucoidan fraction low-density lipoprotein antioxidant cholesteryl ester hydroperoxide |
| 中文关键词: Laminaria japonica fucoidan fraction low-density lipoprotein antioxidant cholesteryl ester hydroperoxide |
| 基金项目: |
| Author Name | Affiliation | E-mail | | LI Laihao | South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China | | | XUE Changhu | Department of Food Science and Technology, Ocean University of China, Qingdao 266003, China | xuech@mail.ouc.edu.cn | | XUE Yong | Department of Food Science and Technology, Ocean University of China, Qingdao 266003, China | | | LI Zhaojie | Department of Food Science and Technology, Ocean University of China, Qingdao 266003, China | | | FU Xueyan | Department of Food Science and Technology, Ocean University of China, Qingdao 266003, China | |
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| Abstract: |
| Five fucoidan fractions from Laminaria japonica with different sulfate content and molecular weight were prepared by anion-exchange chromatography and mild acid hydrolysis. Their antioxidant effects on azo radicals 2-2'-Azobis (2-amidinopropane) dihydrochloride (AAPH) induced oxidation of human low-density lipoprotein (LDL) were evaluated by monitoring cholesteryl ester hydroperoxides (CHL-OOH) formation kinetics through reverse-phase high performance liquid chromatography (RP-HPLC) analysis. Fucoidan F-C with a low molecular mass of 2 000~8 000 and a sulfate content of 24.3% had much stronger protective antioxidant effect than other four fucoidan fractions on the hydrophilic radical AAPH induced LDL oxidation. However, the highly sulfated fucoidan fraction L-B with a molecular mass of 20 000 was completely ineffective in protecting LDL from the AAPH induced oxidation. The results suggested that both molecular mass and sulfate content of fucoidan played very important roles in their effects on the AAPH induced LDL oxidation. |
| 中文摘要: |
| Five fucoidan fractions from Laminaria japonica with different sulfate content and molecular weight were prepared by anion-exchange chromatography and mild acid hydrolysis. Their antioxidant effects on azo radicals 2-2'-Azobis (2-amidinopropane) dihydrochloride (AAPH) induced oxidation of human low-density lipoprotein (LDL) were evaluated by monitoring cholesteryl ester hydroperoxides (CHL-OOH) formation kinetics through reverse-phase high performance liquid chromatography (RP-HPLC) analysis. Fucoidan F-C with a low molecular mass of 2 000~8 000 and a sulfate content of 24.3% had much stronger protective antioxidant effect than other four fucoidan fractions on the hydrophilic radical AAPH induced LDL oxidation. However, the highly sulfated fucoidan fraction L-B with a molecular mass of 20 000 was completely ineffective in protecting LDL from the AAPH induced oxidation. The results suggested that both molecular mass and sulfate content of fucoidan played very important roles in their effects on the AAPH induced LDL oxidation. |
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