| KAN Guangfeng,MIAO Jinlai,SHI Cuijuan,LI Guangyou. 2006. Changes of proteins in the Antarctic ice microalga Chlamydomonas sp.cultured under UV-B radiation stress. Acta Oceanologica Sinica, (2):135－141 |
| Changes of proteins in the Antarctic ice microalga Chlamydomonas sp.cultured under UV-B radiation stress |
| Changes of proteins in the Antarctic ice microalga Chlamydomonas sp.cultured under UV-B radiation stress |
| Received:December 08, 2005 Revised:February 01, 2006 |
| DOI： |
| Key words:Antarctic ice alga Chlamydomonas sp. UV-B radiation 2-DE adaptation mechanisms |
| 中文关键词: Antarctic ice alga Chlamydomonas sp. UV-B radiation 2-DE adaptation mechanisms |
| 基金项目: |
| Author Name | Affiliation | E-mail | | KAN Guangfeng | School of Ocean, Harbin Institute of Technology in Weihai, Weihai 264209, China
State Oceanic Administration Key Laboratory of Marine Bioactive Substances, Qingdao 266061, China | kanguangfeng@163.com | | MIAO Jinlai | State Oceanic Administration Key Laboratory of Marine Bioactive Substances, Qingdao 266061, China | | | SHI Cuijuan | School of Ocean, Harbin Institute of Technology in Weihai, Weihai 264209, China | | | LI Guangyou | State Oceanic Administration Key Laboratory of Marine Bioactive Substances, Qingdao 266061, China | |
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| Abstract: |
| Antarctic ice microalga Chlamydomonas sp.can thrive undisturbed under high UV radiation in the Antarctic ice layer.However, it is unknown that the initial adaptation mechanisms in protein level occurring in response to high UV radiation.Global-expression profiling of proteins in response to stress was analyzed by two-dimensional electrophoresis (2-DE) and image analysis.In the 2-DE analysis, protein preparation is the key step.Three different protein extract methods were compared, and the results showed that the trichloroacetic acid (TCA)-acetone fractional precipitation method was the fittest one.At the same time, the proteins in Chlamydomonas sp.were compared in 2-DE way, and the synthesis of seven protein spots was found disappeared and 18 decreased after exposed to UV-B radiation.In addition, 14 protein spots were enhanced or induced, among which two new peptides (20 and 21 kDa) appeared whose isoelectric point (pI) was 7.05 and 4.60 respectively.These changed proteins might act as key role in the acclimation of Antarctic ice microalga to UV-B radiation. |
| 中文摘要: |
| Antarctic ice microalga Chlamydomonas sp.can thrive undisturbed under high UV radiation in the Antarctic ice layer.However, it is unknown that the initial adaptation mechanisms in protein level occurring in response to high UV radiation.Global-expression profiling of proteins in response to stress was analyzed by two-dimensional electrophoresis (2-DE) and image analysis.In the 2-DE analysis, protein preparation is the key step.Three different protein extract methods were compared, and the results showed that the trichloroacetic acid (TCA)-acetone fractional precipitation method was the fittest one.At the same time, the proteins in Chlamydomonas sp.were compared in 2-DE way, and the synthesis of seven protein spots was found disappeared and 18 decreased after exposed to UV-B radiation.In addition, 14 protein spots were enhanced or induced, among which two new peptides (20 and 21 kDa) appeared whose isoelectric point (pI) was 7.05 and 4.60 respectively.These changed proteins might act as key role in the acclimation of Antarctic ice microalga to UV-B radiation. |
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