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QIN Yingxue,WANG Jun,SU Yongquan,WANG Dexiang,CHEN Xinzhong. 2006. Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara. Acta Oceanologica Sinica, (1):154-159
Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara
Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara
Received:September 03, 2005  Revised:November 28, 2005
DOI:
Key words:Vibrio harveyi  Epinephelus awoara  Ulcer disease
中文关键词:  Vibrio harveyi  Epinephelus awoara  Ulcer disease
基金项目:
Author NameAffiliationE-mail
QIN Yingxue Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China
Fisheries College, Jimei University, Xiamen 361021, China 
 
WANG Jun Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China  
SU Yongquan Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China yqsu@xmu.edu.cn 
WANG Dexiang Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China  
CHEN Xinzhong Department of Oceanography, Institute of Subtropic Ocean, Xiamen University, Xiamen 361005, China
Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China 
 
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Abstract:
      Studies were conducted to determine the cause of the acute mortality of cage-cultured Epinephelus awoara in the Tong'an Bay of Xiamen,China during the summer of 2002.Predominant bacteria strain TS-628 was isolated from the diseased grouper.The virulence test confirmed that TS-628 was the pathogenic bacterium.Biochemical characteristics of the isolates were determined using the automatic bacterial identification system and standard tube tests.To further confirm the identification,a 1 121 bp 16S rRNA gene sequence of the isolate was amplified by PCR,which had been deposited into Genbank (accession number:AY747308).According to the biochemical characteristics and by comparing the 16S rRNA gene homology of the isolate,the pathogenic bacterium was identified as Vibrio harveyi.Drug sensitivity tests showed that this pathogenic bacterium was sensitive to 16 antibacterials,especially to chloramphenicol and actinospectacin,but completely resistant to antibacterials likes vancomycin,penicillin,lincomycin,and so on.
中文摘要:
      Studies were conducted to determine the cause of the acute mortality of cage-cultured Epinephelus awoara in the Tong'an Bay of Xiamen,China during the summer of 2002.Predominant bacteria strain TS-628 was isolated from the diseased grouper.The virulence test confirmed that TS-628 was the pathogenic bacterium.Biochemical characteristics of the isolates were determined using the automatic bacterial identification system and standard tube tests.To further confirm the identification,a 1 121 bp 16S rRNA gene sequence of the isolate was amplified by PCR,which had been deposited into Genbank (accession number:AY747308).According to the biochemical characteristics and by comparing the 16S rRNA gene homology of the isolate,the pathogenic bacterium was identified as Vibrio harveyi.Drug sensitivity tests showed that this pathogenic bacterium was sensitive to 16 antibacterials,especially to chloramphenicol and actinospectacin,but completely resistant to antibacterials likes vancomycin,penicillin,lincomycin,and so on.
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