| LI Huirong,YU Yong,CHEN Bo,ZENG Yinxin,REN Daming. 2005. Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin. Acta Oceanologica Sinica, (6):153－161 |
| Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin |
| Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin |
| Received:July 26, 2005 Revised:October 23, 2005 |
| DOI： |
| Key words:sea ice Arctic Ocean bacteria 16S rDNA phylogenetic analysis |
| 中文关键词: sea ice Arctic Ocean bacteria 16S rDNA phylogenetic analysis |
| 基金项目: |
| Author Name | Affiliation | E-mail | | LI Huirong | State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China | lihuirong72@163.com | | YU Yong | State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China | | | CHEN Bo | State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China | | | ZENG Yinxin | State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China | | | REN Daming | State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China | |
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| Abstract: |
| PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α-proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples. |
| 中文摘要: |
| PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α-proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples. |
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