| TIAN Chao,WANG Guangce,YE Naihao,ZHANG Baoyu,FAN Xiaolei,ZENG Chengkui. 2005. Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides. Acta Oceanologica Sinica, (5):150－161 |
| Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides |
| Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides |
| Received:October 12, 2004 Revised:March 03, 2005 |
| DOI： |
| Key words:rbcL Bryopsis hypnoides sequence analysis phylogenetic analysis codon preference |
| 中文关键词: rbcL Bryopsis hypnoides sequence analysis phylogenetic analysis codon preference |
| 基金项目: |
| Author Name | Affiliation | E-mail | | TIAN Chao | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | | | WANG Guangce | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | gcwang@ms.qdio.ac.cn | | YE Naihao | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | | ZHANG Baoyu | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | | | FAN Xiaolei | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | | | ZENG Chengkui | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | |
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| Abstract: |
| The partial sequence of the rbcL from Bryopsis hypnoides, including the sequences of the upstream, extron and partial intron, was amplified by PCR and their sequences were determined.With Spinacia oleracea as the outgroup, neighbor-joining method and maximum parsimony method were used respectively to build phylogenetic trees according to the rbcL exon sequence among 13species that were the typical species of six phyla.Two kinds of trees showed clearly that there were two groups among those species, the green lineage and the non-green lineage.And the relationships of algae in the green lineage were similar in the two trees but those in the non-green lineage were not consistent.Analysis of codon preference indicated that the codon preference of the rbcL exon of Bryopsis hypnoides distinctly differed from that of the relevant sequence of photosynthetic bacteria. |
| 中文摘要: |
| The partial sequence of the rbcL from Bryopsis hypnoides, including the sequences of the upstream, extron and partial intron, was amplified by PCR and their sequences were determined.With Spinacia oleracea as the outgroup, neighbor-joining method and maximum parsimony method were used respectively to build phylogenetic trees according to the rbcL exon sequence among 13species that were the typical species of six phyla.Two kinds of trees showed clearly that there were two groups among those species, the green lineage and the non-green lineage.And the relationships of algae in the green lineage were similar in the two trees but those in the non-green lineage were not consistent.Analysis of codon preference indicated that the codon preference of the rbcL exon of Bryopsis hypnoides distinctly differed from that of the relevant sequence of photosynthetic bacteria. |
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