| WU Hongqing,TIAN Li,ZHANG Jiuming,SUN Zhaobin,HUANG Leping. 2005. Culture medium optimization for pigment production with RSM method. Acta Oceanologica Sinica, (5):145－149 |
| Culture medium optimization for pigment production with RSM method |
| Culture medium optimization for pigment production with RSM method |
| Received:April 05, 2005 Revised:June 27, 2005 |
| DOI： |
| Key words:response surface methodology red pigment optimization |
| 中文关键词: response surface methodology red pigment optimization |
| 基金项目: |
| Author Name | Affiliation | E-mail | | WU Hongqing | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Department of Oceanic Science and Technology, Hebei University of Technology, Tianjin 300130, China | | | TIAN Li | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Qingdao University of Science and Technology, Qingdao 266042, China | wshw8@yahoo.com.cn | | ZHANG Jiuming | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | SUN Zhaobin | Southwest Forestry College, Kunming 650224, China | | | HUANG Leping | Xinjiang Academy of Agricultural Science, Wulumuqi 83000, China | |
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| Abstract: |
| Response surface methodology was used to optimize a medium for a red-pigmented marine bacterium S-9801 strain (Flavobacterium sp.).In the first optimization step the influence of yeast extract, peptone, glucose and sodium chloride on pigment production was evaluated using a fractional factorial design.Pigment production was positively influenced by glucose and sodium chloride while other components had no significant effect.In the second step the path of steepest ascent was used to approach the optimal region of the medium composition.In the third step the optimal concentration of glucose and sodium chloride was determined by a central composite design and response analysis.The optimized medium allowed pigment production (A535~650) to be increased from 0.137 to 0.559, being 320% higher than the original medium. |
| 中文摘要: |
| Response surface methodology was used to optimize a medium for a red-pigmented marine bacterium S-9801 strain (Flavobacterium sp.).In the first optimization step the influence of yeast extract, peptone, glucose and sodium chloride on pigment production was evaluated using a fractional factorial design.Pigment production was positively influenced by glucose and sodium chloride while other components had no significant effect.In the second step the path of steepest ascent was used to approach the optimal region of the medium composition.In the third step the optimal concentration of glucose and sodium chloride was determined by a central composite design and response analysis.The optimized medium allowed pigment production (A535~650) to be increased from 0.137 to 0.559, being 320% higher than the original medium. |
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