| ZHANG Xichang,LIANG Yubo,FAN Jingfeng,ZHANG Wei,PU Hongyu,LIANG Bin,CHEN Hongxing,SONG Lichao. 2005. Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region. Acta Oceanologica Sinica, (5):139－144 |
| Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region |
| Identification of Perkinsus-like parasite in Manila clam, Ruditapes philippinarum using DNA molecular marker at ITS region |
| Received:February 28, 2005 Revised:June 26, 2005 |
| DOI： |
| Key words:Perkinsus PCR ITS Manila clam |
| 中文关键词: Perkinsus PCR ITS Manila clam |
| 基金项目: |
| Author Name | Affiliation | E-mail | | ZHANG Xichang | Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | weizhang@dicp.ac.cn | | LIANG Yubo | Institute of Marine Environmental Protection, State Oceanic Administration, Dalian 116023, China | | | FAN Jingfeng | Institute of Marine Environmental Protection, State Oceanic Administration, Dalian 116023, China | | | ZHANG Wei | Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | | | PU Hongyu | Life Science and Technology Institute, Dalian Fisheries University, Dalian 116023, China | | | LIANG Bin | Institute of Marine Environmental Protection, State Oceanic Administration, Dalian 116023, China | | | CHEN Hongxing | Institute of Marine Environmental Protection, State Oceanic Administration, Dalian 116023, China | | | SONG Lichao | Institute of Marine Environmental Protection, State Oceanic Administration, Dalian 116023, China | |
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| Abstract: |
| Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China.The internal transcribed spacer(ITS)in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank.The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA(51 bp) and ITS(+5.8 S)(598 bp) regions.The ITS(+5.8S) sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those of Perkinsus atlanticus, and were not more than 95% identical to those of other four Perkinsus species including P.marinus, P.andrewsi, P.andrewsi and P.medierraneus.The ITS (+5.8S) sequence of Perkinsus-like parasite of Shicheng Island was 99% identical to that of Ningbo.These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P.atlanticus, and might reflect P.atlanticus strains of distinct geographic distribution. |
| 中文摘要: |
| Genomic DNA was extracted from hypnospores of Perkinsus-like parasite of Manila clam Ruditapes philippinarum collected at the fishing grounds in Huanghai Sea coast Shicheng Island and East China Sea coast Ningbo, China.The internal transcribed spacer(ITS)in rDNA was PCR-amplified, cloned, sequenced, and compared with that of five Perkinsus species in GenBank.The fragment amplified from DNA of parasite of either Shicheng Island or Ningbo contained 649 bp, including partial ssrRNA(51 bp) and ITS(+5.8 S)(598 bp) regions.The ITS(+5.8S) sequences of Perkinsus-like parasite of both Shicheng Island and Ningbo were all 99% identical to those of Perkinsus atlanticus, and were not more than 95% identical to those of other four Perkinsus species including P.marinus, P.andrewsi, P.andrewsi and P.medierraneus.The ITS (+5.8S) sequence of Perkinsus-like parasite of Shicheng Island was 99% identical to that of Ningbo.These facts about nucleotide sequences suggested that the Perkinsus-like parasite in Manila clam, Ruditapes philippinarum collected from either the Huanghai Sea coast or the East China Sea coast was P.atlanticus, and might reflect P.atlanticus strains of distinct geographic distribution. |
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