| MU Chunhua,BAO Zhenmin,CHEN Gang,HU Jingjie,HAO Lujiang,QI Zizhong,LI Guangxue. 2005. Bacterial diversity in the sediments collected from the Shikoku Basin. Acta Oceanologica Sinica, (3):114－121 |
| Bacterial diversity in the sediments collected from the Shikoku Basin |
| Bacterial diversity in the sediments collected from the Shikoku Basin |
| Received:December 11, 2004 Revised:May 20, 2005 |
| DOI： |
| Key words:bacteria diversity deep-sea sediment Shikoku Basin Northwest Pacific Ocean |
| 中文关键词: bacteria diversity deep-sea sediment Shikoku Basin Northwest Pacific Ocean |
| 基金项目: |
| Author Name | Affiliation | E-mail | | MU Chunhua | Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China | | | BAO Zhenmin | Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China | zmba@ouc.edu.cn | | CHEN Gang | Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China | | | HU Jingjie | Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China | | | HAO Lujiang | Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China | | | QI Zizhong | Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China | | | LI Guangxue | College of Marine Geosciences, Ocean University of China, Qingdao 266003, China | |
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| Abstract: |
| Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups:Proteobacteria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation. |
| 中文摘要: |
| Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups:Proteobacteria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation. |
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