| LIU Chenlin,HUANG Xiaohang,LEE Yookyung,LEE Hongkum,LI Guangyou. 2005. Characteristics and phylogeny of light-harvesting complex gene encoded proteins from marine red alga Griffithsia japonica. Acta Oceanologica Sinica, (2):120－130 |
| Characteristics and phylogeny of light-harvesting complex gene encoded proteins from marine red alga Griffithsia japonica |
| Characteristics and phylogeny of light-harvesting complex gene encoded proteins from marine red alga Griffithsia japonica |
| Received:June 11, 2004 Revised:July 29, 2004 |
| DOI： |
| Key words:Griffithsia japonica light-harvesting gene protein characteristics phylogeny |
| 中文关键词: Griffithsia japonica light-harvesting gene protein characteristics phylogeny |
| 基金项目: |
| Author Name | Affiliation | E-mail | | LIU Chenlin | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Ocean University of China, Qingdao 266003, China | | | HUANG Xiaohang | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | xiaohanghuang@yahoo.ca | | LEE Yookyung | Microbiology Laboratory, Korea Ocean Research & Development Institute(KORDI), Ansan 425-170, Korea | | | LEE Hongkum | Microbiology Laboratory, Korea Ocean Research & Development Institute(KORDI), Ansan 425-170, Korea | | | LI Guangyou | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | |
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| Abstract: |
| Six genes encoding light-harvesting complex (LHC) protein have been characterized in the multicellular red alga Griffithsia japonica EST analysis. Three of them were full sequences while others were partial sequences with 3'-UTRs. The cleavage sites between signal peptide and mature LHC protein were analyzed on these three full sequences. The sequence characteristics, calculated molecular weights and isoelectric point (pI) values and hydrophobieity of the mature proteins were deduced and analyzed. Comparing the LHC sequences of G. japonica with higher plant, Chlorophyta, chromophytes and other red algae, the high conservation of the chlorophyll (Chl) binding site among chromophytes and red algae were revealed. Phylogenetic analysis on LHC proteins from higher plant, green algae, euglena, brown algae, diatom, cryptomonad, Raphidophyte and red algae reveals that (1) there are two distinct groups of Chl a/b and Chl a/c -binding LHC; (2) Chl a binding proteins of red algae share greater similarities with the Chl a/c-binding proteins of the chromophytes and dinoflagellate than with the Chl a/b-binding proteins of the green algae and higher plants; (3) chromophyte's LHC is supposed to be evolved from red algae LHC. |
| 中文摘要: |
| Six genes encoding light-harvesting complex (LHC) protein have been characterized in the multicellular red alga Griffithsia japonica EST analysis. Three of them were full sequences while others were partial sequences with 3'-UTRs. The cleavage sites between signal peptide and mature LHC protein were analyzed on these three full sequences. The sequence characteristics, calculated molecular weights and isoelectric point (pI) values and hydrophobieity of the mature proteins were deduced and analyzed. Comparing the LHC sequences of G. japonica with higher plant, Chlorophyta, chromophytes and other red algae, the high conservation of the chlorophyll (Chl) binding site among chromophytes and red algae were revealed. Phylogenetic analysis on LHC proteins from higher plant, green algae, euglena, brown algae, diatom, cryptomonad, Raphidophyte and red algae reveals that (1) there are two distinct groups of Chl a/b and Chl a/c -binding LHC; (2) Chl a binding proteins of red algae share greater similarities with the Chl a/c-binding proteins of the chromophytes and dinoflagellate than with the Chl a/b-binding proteins of the green algae and higher plants; (3) chromophyte's LHC is supposed to be evolved from red algae LHC. |
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