| QIU Lihua,SONG Linsheng,WU Longtao,XU Wei,JIANG Shigui. 2005. cDNA cloning and mRNA expression of the translationally controlled tumor protein (TCTP) gene from Japanese sea perch (Lateolabrax japonicus). Acta Oceanologica Sinica, (2):113－119 |
| cDNA cloning and mRNA expression of the translationally controlled tumor protein (TCTP) gene from Japanese sea perch (Lateolabrax japonicus) |
| cDNA cloning and mRNA expression of the translationally controlled tumor protein (TCTP) gene from Japanese sea perch (Lateolabrax japonicus) |
| Received:June 11, 2004 Revised:August 20, 2004 |
| DOI： |
| Key words:translationally controlled tumor protein Lateolabrax japonicus cDNA cloning mRNA expression |
| 中文关键词: translationally controlled tumor protein Lateolabrax japonicus cDNA cloning mRNA expression |
| 基金项目: |
| Author Name | Affiliation | E-mail | | QIU Lihua | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
Graduate School, Chinese Academy of Sciences, Beijing 100039, China | | | SONG Linsheng | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | lshsong@ms.qdio.ac.cn | | WU Longtao | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate School, Chinese Academy of Sciences, Beijing 100039, China | | | XU Wei | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | JIANG Shigui | South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China | |
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| Abstract: |
| A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that ofzebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver. |
| 中文摘要: |
| A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that ofzebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver. |
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