| ZHOU Hantao,GAO Yuanyuan,CHEN Xu,YE Fan,LIAN Yuwu. 2005. Study on genetic diversity and resource conservation of amphioxus (Branchiostoma balcheri Gray) population. Acta Oceanologica Sinica, (2):102－112 |
| Study on genetic diversity and resource conservation of amphioxus (Branchiostoma balcheri Gray) population |
| Study on genetic diversity and resource conservation of amphioxus (Branchiostoma balcheri Gray) population |
| Received:April 11, 2004 Revised:December 02, 2004 |
| DOI： |
| Key words:Branchiostoma belcheri Gray genetic diversity molecular markers ISSRs RAPDs genetic conservation |
| 中文关键词: Branchiostoma belcheri Gray genetic diversity molecular markers ISSRs RAPDs genetic conservation |
| 基金项目: |
| Author Name | Affiliation | E-mail | | ZHOU Hantao | School of Life Sciences, Xiamen University, Xiamen 361005, China
Key Laboratory of the Ministry of Education for Cell Biological Tum or Cell Engineering, Xiamen Uinversity, Xiamen 361005, China | htzhou@jingxian.xmu.edu.cn | | GAO Yuanyuan | School of Life Sciences, Xiamen University, Xiamen 361005, China | | | CHEN Xu | School of Life Sciences, Xiamen University, Xiamen 361005, China | | | YE Fan | School of Life Sciences, Xiamen University, Xiamen 361005, China | | | LIAN Yuwu | School of Life Sciences, Xiamen University, Xiamen 361005, China | |
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| Abstract: |
| Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates.Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNAs (RAPDs) technologies were applied to detect the genetic variation of 3 bulking samples and individually sampled populations in nowadays Xiamen sea areas (Xiekou, Nanxian and Huangcuo) where the amphioxuses are alive. For the bulking sampled populations, 5 ISSR and 10 RAPD primers generated 357bands, of which 181 (50.7%) were polymorphic. Nei index and UPGMA statistical analysis indicated that amphioxuses in these 3areas could be divided into 2 groups. The genetic distance between animals in Nanxian and Huangcuo areas was 0.07 and classified into 1 group, while the population in Xiekou belonged to another group because its genetic distances in Nanxian and Huangcuo were 0.12 and 0.14, respectively. The result was in accordance with the morphological comparison among animals from those areas. For individually sampled population, Shannon's index of genetic diversity was used to partition the diversity of the animals among these 3sea areas, and the results showed that the indices in populations of Xiekou, Nanxian and Huangcuo, were 0.583, 0.482 and 0.374,respectively. The linear regression equation analysis for amphioxus' genetic diversity versus the environment factors revealed that granularity/sorting coefficient and water depth were the most important factors that affect amphioxus genetic diversity. On the basis of the results, the suggestions for amphioxus resource conservation in Xiamen sea areas are put forward. |
| 中文摘要: |
| Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates.Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNAs (RAPDs) technologies were applied to detect the genetic variation of 3 bulking samples and individually sampled populations in nowadays Xiamen sea areas (Xiekou, Nanxian and Huangcuo) where the amphioxuses are alive. For the bulking sampled populations, 5 ISSR and 10 RAPD primers generated 357bands, of which 181 (50.7%) were polymorphic. Nei index and UPGMA statistical analysis indicated that amphioxuses in these 3areas could be divided into 2 groups. The genetic distance between animals in Nanxian and Huangcuo areas was 0.07 and classified into 1 group, while the population in Xiekou belonged to another group because its genetic distances in Nanxian and Huangcuo were 0.12 and 0.14, respectively. The result was in accordance with the morphological comparison among animals from those areas. For individually sampled population, Shannon's index of genetic diversity was used to partition the diversity of the animals among these 3sea areas, and the results showed that the indices in populations of Xiekou, Nanxian and Huangcuo, were 0.583, 0.482 and 0.374,respectively. The linear regression equation analysis for amphioxus' genetic diversity versus the environment factors revealed that granularity/sorting coefficient and water depth were the most important factors that affect amphioxus genetic diversity. On the basis of the results, the suggestions for amphioxus resource conservation in Xiamen sea areas are put forward. |
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