| WANG Jun,ZHANG Wen,SU Yongquan,DING Shaoxiong. 2004. Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences. Acta Oceanologica Sinica, (4):709－716 |
| Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences |
| Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences |
| Received:October 11, 2003 Revised:January 20, 2004 |
| DOI： |
| Key words:S rRNA Neobenedenia sequence analysis |
| 中文关键词: S rRNA Neobenedenia sequence analysis |
| 基金项目: |
| Author Name | Affiliation | E-mail | | WANG Jun | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China | junw@xmu.edu.cn | | ZHANG Wen | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China | | | SU Yongquan | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China | | | DING Shaoxiong | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China | |
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| Abstract: |
| The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda,Neobenedenia girellae and N.melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced.The results show that the comparison of 28S rRNA sequences,with only a base varying in 337bp accounting for 0.3% genetic difference,from the relative species N.girellae and N.melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%,higher than that of 99.41% for the single species N.melleni sampled in different areas,and the intraspecific divergence of N.melleni is 0.59%.Meanwhile,the interspecific differences between the two Neobenedenia and three Benedenia (i.e.,B.lutjani,B.rohdei and B.seriolae) range from 2.08% to11.73%.In addition,UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other.Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity,whether they belong to a single species or not are still undefined,and the more genes of them should be further investigated,in combination with the systematical and detailed morphological study. |
| 中文摘要: |
| The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda,Neobenedenia girellae and N.melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced.The results show that the comparison of 28S rRNA sequences,with only a base varying in 337bp accounting for 0.3% genetic difference,from the relative species N.girellae and N.melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%,higher than that of 99.41% for the single species N.melleni sampled in different areas,and the intraspecific divergence of N.melleni is 0.59%.Meanwhile,the interspecific differences between the two Neobenedenia and three Benedenia (i.e.,B.lutjani,B.rohdei and B.seriolae) range from 2.08% to11.73%.In addition,UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other.Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity,whether they belong to a single species or not are still undefined,and the more genes of them should be further investigated,in combination with the systematical and detailed morphological study. |
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