| Lin Xuezheng,Yang Xiuxia,Bian Ji,Huang Xiaohang. 2003. Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean. Acta Oceanologica Sinica, (4):643－649 |
| Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean |
| Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean |
| Received:March 18, 2003 Revised:August 12, 2003 |
| DOI： |
| Key words:Southern Ocean deep sea psychrophilic bacteria low-temperature lipase |
| 中文关键词: Southern Ocean deep sea psychrophilic bacteria low-temperature lipase |
| 基金项目:This study was supported by 10th five year plan(DY105040203) of China Ocean Mineral Resources R&D Association |
| Author Name | Affiliation | | Lin Xuezheng | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | Yang Xiuxia | Fishery College, Ocean University of China, Qingdao 266003, China | | Bian Ji | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | Huang Xiaohang | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China |
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| Abstract: |
| A strain of psychrophilic bacterium,2-5-10-1,which produces low-temperature lipase,is isolated from the deep sea of Prydz Bay in Southern Ocean.The highest lipase secretion of this strain is observed at 5℃ and this temperature is also for optimal growth.Tween 80 and olive oil enhance secretion of lipase.The optimal temperature and pH for lipase activity are 35℃ and 7.5℃ respectively.At 0℃,the lipase still has 37% relative enzyme activity.The lipase shows high thermolability,more than 50% activity lost after incubation at 60℃ for 15 min.EDTA has no effect on lipase activity,indicating the lipase activity is independent of divalent cation.In contrast,the lipase activity is inhibited drastically by Cu2+ and Zn2+. |
| 中文摘要: |
| A strain of psychrophilic bacterium,2-5-10-1,which produces low-temperature lipase,is isolated from the deep sea of Prydz Bay in Southern Ocean.The highest lipase secretion of this strain is observed at 5℃ and this temperature is also for optimal growth.Tween 80 and olive oil enhance secretion of lipase.The optimal temperature and pH for lipase activity are 35℃ and 7.5℃ respectively.At 0℃,the lipase still has 37% relative enzyme activity.The lipase shows high thermolability,more than 50% activity lost after incubation at 60℃ for 15 min.EDTA has no effect on lipase activity,indicating the lipase activity is independent of divalent cation.In contrast,the lipase activity is inhibited drastically by Cu2+ and Zn2+. |
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