| Wang Bin,Jia Jianhang,Shi Jinfeng,Chen Yihua,Jin Demin,Xu Pu,Mei Junxue,Weng Manli. 2001. Identification of Porphyra lines using computerized DNA fingerprinting. Acta Oceanologica Sinica, (3):401－407 |
| Identification of Porphyra lines using computerized DNA fingerprinting |
| Identification of Porphyra lines using computerized DNA fingerprinting |
| Received:December 12, 2000 Revised:February 02, 2001 |
| DOI： |
| Key words:Computerized DNA fingerprinting RAPD Porphyra germplasm identification |
| 中文关键词: Computerized DNA fingerprinting RAPD Porphyra germplasm identification |
| 基金项目:This study was supported by China Ocean "863" Project;National Natural Science Foundation of China. |
| Author Name | Affiliation | E-mail | | Wang Bin | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China | | | Jia Jianhang | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China | | | Shi Jinfeng | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China | | | Chen Yihua | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China | | | Jin Demin | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China | | | Xu Pu | Jiangsu Marine Fishery Institute, Nantong 226007, China | | | Mei Junxue | Institute of Oceanolagy, Chinese Academy of Sciences, Qingdao 266071, China | | | Weng Manli | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China | mlweng@genetics.ac.cn |
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| Abstract: |
| RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P.yezoensis,P.haitanensis,P.katadai var.Hemiphylla and P.oligospermatangia:Eight stable and repeatable RAPD bands amplified with two primers,OPN-02 and OPJ-18,were selected for the construction of DNA fingerprinting.The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals,1 and 0,which represented the presence (numbered as 1) or absence (numbered as 0) of each band,respectively.Based on these results,a model DNA fingerprint and a computerized DNA fingerprint were constructed.In the constructed DNA fingerprint,each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other.Later,a software,named as PhGI,was designed based on this DNA fingerprinting.It can be used in practical Porphyra line identification. |
| 中文摘要: |
| RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P.yezoensis,P.haitanensis,P.katadai var.Hemiphylla and P.oligospermatangia:Eight stable and repeatable RAPD bands amplified with two primers,OPN-02 and OPJ-18,were selected for the construction of DNA fingerprinting.The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals,1 and 0,which represented the presence (numbered as 1) or absence (numbered as 0) of each band,respectively.Based on these results,a model DNA fingerprint and a computerized DNA fingerprint were constructed.In the constructed DNA fingerprint,each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other.Later,a software,named as PhGI,was designed based on this DNA fingerprinting.It can be used in practical Porphyra line identification. |
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