| Zhou Zhigang,Li Dapeng,Wu Chaoyuan,Liu Haihang. 2000. Laminaria gametophyte clone culture and its application in sporeling cultivation. Acta Oceanologica Sinica, (2):89－95 |
| Laminaria gametophyte clone culture and its application in sporeling cultivation |
| Laminaria gametophyte clone culture and its application in sporeling cultivation |
| Received:September 06, 1998 Revised:April 18, 1999 |
| DOI： |
| Key words:Clone culture gametophyte Laminaria cultivation nitrate effect phosphate effect sporeling sporophyte |
| 中文关键词: Clone culture gametophyte Laminaria cultivation nitrate effect phosphate effect sporeling sporophyte |
| 基金项目:This research was supported by the Key Project, the Ninth Five-Year Plan of China 96-008-01-02-06,and is a part of the Project of Bio-Engineering Center of China, SSTC 96-C01-05-01 as well. |
| Author Name | Affiliation | | Zhou Zhigang | College of Fishery Science, Shanghai Fisheries University, 334 Jungong Road, Shangha 200090, China | | Li Dapeng | Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China | | Wu Chaoyuan | Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China | | Liu Haihang | Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China |
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| Abstract: |
| In China,the total annual production of cultured Laminaria japonica reached half million tons in dry weight these few years.The routine sporeling culture technique conducted in the greenhouse took aF least three and half months.In such a case,sometimes the sporelings died within a few days due to destructive diseases.In order to overcome the mentioned problems,a new sporeling culture technique,the clone technique,is developed.The method includes three steps:(1)Gametophyte clone culture.The spores and the gametophytes are cloned in flasks under favorable environments.(2) Sporeling cultore.Male and female clones are crushed and spread onto a frame to allow the gametophytes to attach to the substrata.The frames are cultured in tanks,and the sporophytes reach 1 cm in length within one and a half months.(3) Outgrowing of the plant.The frames are put in the open sea when seawater temperature decreased to 20℃.After one month,the sporelings are large enough to be transplanted.It is concluded that the clone technique has the following advantages:(1) Large amount of clones can be produced in a short period of time.(2) Clone seeding method makes it free from the biological rhythm.one can seed the plant anytime all the year round.(3) It takes only one and a half months to complete the process of sporeling cultivation in the greenhouse.At present,this technique is used in the breeding of new strains of Laminoria. |
| 中文摘要: |
| In China,the total annual production of cultured Laminaria japonica reached half million tons in dry weight these few years.The routine sporeling culture technique conducted in the greenhouse took aF least three and half months.In such a case,sometimes the sporelings died within a few days due to destructive diseases.In order to overcome the mentioned problems,a new sporeling culture technique,the clone technique,is developed.The method includes three steps:(1)Gametophyte clone culture.The spores and the gametophytes are cloned in flasks under favorable environments.(2) Sporeling cultore.Male and female clones are crushed and spread onto a frame to allow the gametophytes to attach to the substrata.The frames are cultured in tanks,and the sporophytes reach 1 cm in length within one and a half months.(3) Outgrowing of the plant.The frames are put in the open sea when seawater temperature decreased to 20℃.After one month,the sporelings are large enough to be transplanted.It is concluded that the clone technique has the following advantages:(1) Large amount of clones can be produced in a short period of time.(2) Clone seeding method makes it free from the biological rhythm.one can seed the plant anytime all the year round.(3) It takes only one and a half months to complete the process of sporeling cultivation in the greenhouse.At present,this technique is used in the breeding of new strains of Laminoria. |
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