| Guo Baotai,Dai Jixun,Shen Songdong,Bi Yuping,Shan Lei,Li Guangcun. 2000. Extraction of plasmid-like DNA and high-quality total DNA from Porphyra yezoensis. Acta Oceanologica Sinica, (2):83－88 |
| Extraction of plasmid-like DNA and high-quality total DNA from Porphyra yezoensis |
| Extraction of plasmid-like DNA and high-quality total DNA from Porphyra yezoensis |
| Received:January 10, 2000 Revised:February 15, 2000 |
| DOI： |
| Key words:Porphyra yezoensis somatic cells total DNA glassmilk restriction digestion plasmid like DNA |
| 中文关键词: Porphyra yezoensis somatic cells total DNA glassmilk restriction digestion plasmid like DNA |
| 基金项目: |
| Author Name | Affiliation | | Guo Baotai | College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China | | Dai Jixun | College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China | | Shen Songdong | College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China | | Bi Yuping | Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100 | | Shan Lei | Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100 | | Li Guangcun | Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100 |
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| Abstract: |
| Somatic cells were prepared from sea snail enzyme digests of Porphyra yezoensis thalli.Using SDS-Proteinase K as extraction solution,total DNA was isolated from the somatic cells.The crude extracts of total DNA were purified with glassmilk,and the resulting DNA was of sufficient quality for digestion of restriction endonuclease.DNA bands were clearly observed in the restriction patterns of EcoRI,PstI and HaeⅢ respectively.The presence of DNA bands in the restriction pattern of total DNA indicated that the genome of Porphyra yezoensis may be small.Unexpectedly,using guanidinium isothiocyanate and sarcosyl as extraction solution,a plasmid-like DNA band(2.3 Kb) was directly found in the isolated total DNA of Porphyra yezoensis.A very simple and convenient method for plasmid-like DNA isolation has been established. |
| 中文摘要: |
| Somatic cells were prepared from sea snail enzyme digests of Porphyra yezoensis thalli.Using SDS-Proteinase K as extraction solution,total DNA was isolated from the somatic cells.The crude extracts of total DNA were purified with glassmilk,and the resulting DNA was of sufficient quality for digestion of restriction endonuclease.DNA bands were clearly observed in the restriction patterns of EcoRI,PstI and HaeⅢ respectively.The presence of DNA bands in the restriction pattern of total DNA indicated that the genome of Porphyra yezoensis may be small.Unexpectedly,using guanidinium isothiocyanate and sarcosyl as extraction solution,a plasmid-like DNA band(2.3 Kb) was directly found in the isolated total DNA of Porphyra yezoensis.A very simple and convenient method for plasmid-like DNA isolation has been established. |
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