| Liu Ping,Kong Jie,Shi Tuo,Zhuang Zhimeng,Deng Jingyao. 2000. RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis) in Chinese coastal waters of the Huanghai Sea and coastal waters of the Bohai Sea. Acta Oceanologica Sinica, (1):119－126 |
| RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis) in Chinese coastal waters of the Huanghai Sea and coastal waters of the Bohai Sea |
| RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis) in Chinese coastal waters of the Huanghai Sea and coastal waters of the Bohai Sea |
| Received:June 30, 1999 Revised:October 08, 1999 |
| DOI： |
| Key words:Penaeus chinensis stock of the Huanghai and Bohai Seas RAPD technique genetic diversity |
| 中文关键词: Penaeus chinensis stock of the Huanghai and Bohai Seas RAPD technique genetic diversity |
| 基金项目:This study is supported by the National Natural Science Foundation of China finder the Project No. 39620260. |
| Author Name | Affiliation | | Liu Ping | Huanghai Sea Fisheries Research institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | Kong Jie | Huanghai Sea Fisheries Research institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | Shi Tuo | Huanghai Sea Fisheries Research institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | Zhuang Zhimeng | Huanghai Sea Fisheries Research institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | Deng Jingyao | Huanghai Sea Fisheries Research institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| Abstract: |
| Genetic diversity of 32 individuals of P.chinensis in the Chinese coastal waters of the Huanghai and Bohai Seas was detected by RAPD technique.Twenty decamer primers of OPI were used for DNA amplification for each individual.The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty-nine of 106 loci detected were polymorphic, amounting to 36.8%.Mean genetic distance was 0.094 1±0.020 6;68 markers (63.2% of the total) showed stable homogeneity in all of the 32 individuals. |
| 中文摘要: |
| Genetic diversity of 32 individuals of P.chinensis in the Chinese coastal waters of the Huanghai and Bohai Seas was detected by RAPD technique.Twenty decamer primers of OPI were used for DNA amplification for each individual.The results showed that: Seventeen primers obtained reproducible fingerprints, and the bands were clear. Thirty-nine of 106 loci detected were polymorphic, amounting to 36.8%.Mean genetic distance was 0.094 1±0.020 6;68 markers (63.2% of the total) showed stable homogeneity in all of the 32 individuals. |
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