| Song Siyang,Luo Wenxin,Chen Jin'an,Shen Mingshan,Su Wenjin. 1999. In situ enzyme immunoassays with tissue smears to study horizontal transmission of BMNV in penaeid shrimp and in beach animals. Acta Oceanologica Sinica, (1):109－116 |
| In situ enzyme immunoassays with tissue smears to study horizontal transmission of BMNV in penaeid shrimp and in beach animals |
| In situ enzyme immunoassays with tissue smears to study horizontal transmission of BMNV in penaeid shrimp and in beach animals |
| Received:December 25, 1997 Revised:January 28, 1998 |
| DOI： |
| Key words:Enzyyne immunoassays BMNV penaeid shrimp beach animals |
| 中文关键词: Enzyyne immunoassays BMNV penaeid shrimp beach animals |
| 基金项目:This study was supported by the Xiamen City Science and Technology Commission, Fujian Province. |
| Author Name | Affiliation | | Song Siyang | Department of Biology, Xiamen University, Xiamen 361005, China | | Luo Wenxin | Department of Biology, Xiamen University, Xiamen 361005, China | | Chen Jin'an | Department of Biology, Xiamen University, Xiamen 361005, China | | Shen Mingshan | Department of Biology, Xiamen University, Xiamen 361005, China | | Su Wenjin | Department of Biology, Xiamen University, Xiamen 361005, China |
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| Abstract: |
| An enzyme immunoassay technique is developed for detecting the baculoviral midgut gland necrosis virus (BMNV) in penaeid shrimp and beach animals. An antiserum was obtained from rabbits immunized with a BMNV purified from Penaeus japnnicus.γ-globulin was isolated and labelled with horseradish peroxidase (HRP).This reagent was used to detect the virus in penaeid shrimp experimentally infected with the BMNV preparalion, in cultured penaeid shrimp and in several beach animals. The technique is specific, simple and sensitive and can be developed into a practical method for the detection of penaeid shrimp viruses. Among 128 tissue samples from 11 beach animals, 77 samples gave positive reactions with the enzyme-labelled immunoglobulin against BMNV from P. japonicas. These animals could possibly constitute a horizontal transmission pathway for cultured penaeid shrimp vcruses. |
| 中文摘要: |
| An enzyme immunoassay technique is developed for detecting the baculoviral midgut gland necrosis virus (BMNV) in penaeid shrimp and beach animals. An antiserum was obtained from rabbits immunized with a BMNV purified from Penaeus japnnicus.γ-globulin was isolated and labelled with horseradish peroxidase (HRP).This reagent was used to detect the virus in penaeid shrimp experimentally infected with the BMNV preparalion, in cultured penaeid shrimp and in several beach animals. The technique is specific, simple and sensitive and can be developed into a practical method for the detection of penaeid shrimp viruses. Among 128 tissue samples from 11 beach animals, 77 samples gave positive reactions with the enzyme-labelled immunoglobulin against BMNV from P. japonicas. These animals could possibly constitute a horizontal transmission pathway for cultured penaeid shrimp vcruses. |
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