| Wang Bin,Yang Feng,Chen Rongzhong,Xu Xun. 1997. Detection of Hepatitis A virus in shellfish by reverse transcription PCR. Acta Oceanologica Sinica, (4):527－535 |
| Detection of Hepatitis A virus in shellfish by reverse transcription PCR |
| Detection of Hepatitis A virus in shellfish by reverse transcription PCR |
| Received:October 20, 1996 Revised:January 05, 1997 |
| DOI： |
| Key words:Shellfish HAV RT-PCR competitive |
| 中文关键词: Shellfish HAV RT-PCR competitive |
| 基金项目: |
| Author Name | Affiliation | | Wang Bin | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Yang Feng | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Chen Rongzhong | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Xu Xun | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: |
| A reverse transcription PCR (RT-PCR) method was used to detect the Hepatitis A virus in clam tissues The RNA template for PCR was prepared by a modified described previously (Yang and Xu, 1993).Its recovery wes determined by DNA hybridization.The sensitivity of RT-PCR and the quantitation of HAV were determined by quantitative competitive PCR. |
| 中文摘要: |
| A reverse transcription PCR (RT-PCR) method was used to detect the Hepatitis A virus in clam tissues The RNA template for PCR was prepared by a modified described previously (Yang and Xu, 1993).Its recovery wes determined by DNA hybridization.The sensitivity of RT-PCR and the quantitation of HAV were determined by quantitative competitive PCR. |
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