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Zhang Xuecheng,Wu Xiaonan,Li Yonghong. 1995. The effects of immobilization on physiology and ultrastructure of unicellular alga Platymonas subcordiformis. Acta Oceanologica Sinica, (2):269－275
The effects of immobilization on physiology and ultrastructure of unicellular alga Platymonas subcordiformis
The effects of immobilization on physiology and ultrastructure of unicellular alga Platymonas subcordiformis
Received:January 20, 1995  Revised:April 20, 1995
DOI：
Key words:Platymonas subcordiformis  immobilization  photosynthesis  respiration  ultrastructure
中文关键词:  Platymonas subcordiformis  immobilization  photosynthesis  respiration  ultrastructure
基金项目:
Author NameAffiliation
Zhang Xuecheng College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China 
Wu Xiaonan Institute of Oceanology, Academia Sinica, Qingdao 266071, China 
Li Yonghong College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China 
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Abstract:
      In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0.5, 0.75, 1.0, 1.5 and 2.0 mol/dm3), cultured at 15℃ and 25℃.The growth rate and morphology were studied and photosynthesis and respiration rates were measured.The optimum conditions of immobilized culture is in 0.5 mol/dm3 of CaCl2, at 25℃.Photosynthesis rate of immobilized cells is higher than free cultivated cells, and that at 15℃ than at 25℃.Under scanning electropic microscope, it was shown that the immobilized cells had larger size and rougher surface.The relationship between growth of immobilized cells and abiotic factors and the prospects of applying immobilized cultivation are also discussed.
中文摘要:
      In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0.5, 0.75, 1.0, 1.5 and 2.0 mol/dm3), cultured at 15℃ and 25℃.The growth rate and morphology were studied and photosynthesis and respiration rates were measured.The optimum conditions of immobilized culture is in 0.5 mol/dm3 of CaCl2, at 25℃.Photosynthesis rate of immobilized cells is higher than free cultivated cells, and that at 15℃ than at 25℃.Under scanning electropic microscope, it was shown that the immobilized cells had larger size and rougher surface.The relationship between growth of immobilized cells and abiotic factors and the prospects of applying immobilized cultivation are also discussed.
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