| Gao Hongfeng,Cao Wenda,Ji Minghou. 1994. Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis. Acta Oceanologica Sinica, (2):253－259 |
| Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis |
| Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis |
| Received:February 26, 1993 Revised:January 08, 1994 |
| DOI： |
| Key words:Porphyra haitanensis R-phycocyanin allophycocyanin subunit and chromophore |
| 中文关键词: Porphyra haitanensis R-phycocyanin allophycocyanin subunit and chromophore |
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| Abstract: |
R-phycocyarnin (RPC) of Porphyra haitmmsis (T.J.Chang et B.F.Zheng) was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as an eluent, and α and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respectively, while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respeMively, and those of RPC and APC were at 117 000 and 122 000, respectively.
Both the molar ratio of α and β subunits of RPC and APC were 1:1, and the subunit composition was conf firmed to be(αβ)3.
It was ascertained that in RPC a subunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB),while in APC both α and β subunits contain one PCB. |
| 中文摘要: |
R-phycocyarnin (RPC) of Porphyra haitmmsis (T.J.Chang et B.F.Zheng) was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as an eluent, and α and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respectively, while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respeMively, and those of RPC and APC were at 117 000 and 122 000, respectively.
Both the molar ratio of α and β subunits of RPC and APC were 1:1, and the subunit composition was conf firmed to be(αβ)3.
It was ascertained that in RPC a subunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB),while in APC both α and β subunits contain one PCB. |
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