| Yang Feng,Xu Xun,Zhang Yusheng,Chen Rongzhong,Zhou Xiulan. 1993. A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction. Acta Oceanologica Sinica, (2):279－284 |
| A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction |
| A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction |
| Received:April 15, 1992 Revised:June 03, 1992 |
| DOI： |
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| Author Name | Affiliation | | Yang Feng | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Xu Xun | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Zhang Yusheng | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Chen Rongzhong | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Zhou Xiulan | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: |
| Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test. |
| 中文摘要: |
| Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test. |
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