| HAN Yinglun,LI Jun,XU Lei,MA Qinghua,GOU Meng,PANG Yue,LIU Xin,LI Qingwei. 0. Molecular cloning,expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica. Acta Oceanologica Sinica, (0):0－ |
| Molecular cloning,expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica |
| Molecular cloning,expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica |
| Received:March 13, 2016 Revised:April 28, 2017 |
| DOI：10.1007/s13131-017-1095-y |
| 中文关键词: Guanine nucleotide exchange factor Vav2|Lampetra japonica|phylogenetic relationship|Molecular cloning |
| 英文关键词:Guanine nucleotide exchange factor Vav2|Lampetra japonica|phylogenetic relationship|Molecular cloning |
| 基金项目:The National Program on Key Basic Research Project (973 Program) under contract No.2013CB835304;the National Marine Public Projects under contract No.201305016; the National Natural Science Foundation of China (General Program) under contract No. 31601865;Dalian Science and Technology Program under contract No.2013E11SF056;the Natural Science Foundation of Liaoning Normal University under contract No.LS2015L010. |
| Author Name | Affiliation | E-mail | | HAN Yinglun | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | LI Qingwei,E-mail:liqw@263.net | | LI Jun | College of Life Science, Liaoning Normal University, Dalian 116029, China
Freshwater Fisheries Sciences Institute of Liaoning Province, Liaoyang 111000, China | | | XU Lei | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | | | MA Qinghua | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | | | GOU Meng | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | | | PANG Yue | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | | | LIU Xin | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | | | LI Qingwei | College of Life Science, Liaoning Normal University, Dalian 116029, China
Lamprey Research Center, Liaoning Normal University, Dalian 116029, China | liqw@263.net |
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| 中文摘要: |
| The Guanine nucleotide exchange factor Vav2(Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases.In the current study,an ortholog (Lj-Vav2) of Vav2 was identified in the lamprey (Lampetra japonica).To elucidated the phylogenetic relationship of Vav2,the metazoan genome databases were analyzed to mine the ortholog of Vav.It was found that Vav2 genes were only existed in vertebrates and Lj-Vav2 was the original one found in agnathans.The evolutionary dynamics of conserved motifs of Vav2 were explored using combined amino acid sequence as markers,and it is revealed that the Calponin homology (CH) domain, Dbl-homologous (DH) domain,Pleckstrin homology (PH) domain,Cysteine-rich (C1) domains,Srchomology 3(SH3) domains and Src homology 2(SH2) domain were conserved throughout the Vav2 gene family in vertebrates during gene evolution.Relative quantitative real-time PCR analysis showed that the Lj-Vav2 was distributed in the heart,kidney,supraneural myeloid body,liver,gill and lymphocyte-like cells.The Lj-Vav2 was found to be expressed in these tissues,and the level of which was upregulated in lymphocyte-like cells after the animal was stimulated with LPS.These results indicated that the Lj-Vav2 might be involved in the immune response of lymphocyte-like cells in lamprey.Meanwhile,our findings provided a foundation for further investigation of the function of Lj-Vav2 in the primary vertebrate. |
| 英文摘要: |
| The Guanine nucleotide exchange factor Vav2(Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases.In the current study,an ortholog (Lj-Vav2) of Vav2 was identified in the lamprey (Lampetra japonica).To elucidated the phylogenetic relationship of Vav2,the metazoan genome databases were analyzed to mine the ortholog of Vav.It was found that Vav2 genes were only existed in vertebrates and Lj-Vav2 was the original one found in agnathans.The evolutionary dynamics of conserved motifs of Vav2 were explored using combined amino acid sequence as markers,and it is revealed that the Calponin homology (CH) domain, Dbl-homologous (DH) domain,Pleckstrin homology (PH) domain,Cysteine-rich (C1) domains,Srchomology 3(SH3) domains and Src homology 2(SH2) domain were conserved throughout the Vav2 gene family in vertebrates during gene evolution.Relative quantitative real-time PCR analysis showed that the Lj-Vav2 was distributed in the heart,kidney,supraneural myeloid body,liver,gill and lymphocyte-like cells.The Lj-Vav2 was found to be expressed in these tissues,and the level of which was upregulated in lymphocyte-like cells after the animal was stimulated with LPS.These results indicated that the Lj-Vav2 might be involved in the immune response of lymphocyte-like cells in lamprey.Meanwhile,our findings provided a foundation for further investigation of the function of Lj-Vav2 in the primary vertebrate. |
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